Evaluation of in vitro irradiation setup: Designed for the horizontal beamline at the Danish Centre for Particle Therapy
DOI:
https://doi.org/10.2340/1651-226X.2024.19657Keywords:
Radiobiology, proton therapy, water-phantom, Clonogenic assays, irradiation modalitiesAbstract
Background: Radiobiological experimental setups are challenged by precise sample positioning along depth dose profile, scattering conditions, and practical difficulties that must be addressed in individual designs.
The aim of this study was to produce cell survival curves with several irradiation modalities, by using a setup designed at the Danish Centre for Particle Therapy (DCPT) for in vitro proton irradiations using a horizontal beam line and thereby evaluating the setups use for in vitro irradiations experiments.
Materials and methods: The setup is a water phantom suitable for in vitro research with multiple irradiation modalities, in particular the pencil scanning proton beam available from a horizontal experimental beamline. The phantom included a water tank of 39.0 × 17.0 × 20.5 cm. Cell survival-curves were produced using the cell line V79 Chinese hamster lung fibroblast cells (V79s) in biological triplicates of clonogenic assays. Cell survival curves were produced with both a 18 MeV electron beam, 6 MV photon beam, and a Spread-Out Bragg Peak (SOBP) proton beam formed by pristine energies of 85–111 MeV where three positions were examined.
Results: Survival curves with uncertainty areas were made for all modalities. Dosimetric uncertainty amounted to, respectively, 4%, 3% and 3% for proton, electron, and high energy photon irradiations. Cell survival fraction uncertainty was depicted as the standard deviation between replications of the experiment.
Conclusion: Cell survival curves could be produced with acceptable uncertainties using this novel water phantom and cellular laboratory workflow. The setup is useful for future in vitro irradiation experiments.
Downloads
References
Overgaard CB, Reaz F, Sitarz M, et al. An experimental setup for proton irradiation of a murine leg model for radiobiological studies. Acta Oncol. 2023;62(11):1566–73. DOI: https://doi.org/10.1080/0284186X.2023.2246641
Sørensen BS, Vestergaard A, Overgaard J, Præstegaard LH. Dependence of cell survival on instantaneous dose rate of a linear accelerator. Radiother Oncol. 2011;101(1):223–5. https://doi.org/10.1016/j.radonc.2011.06.018 DOI: https://doi.org/10.1016/j.radonc.2011.06.018
Biglin ER, Aitkenhead AH, Price GJ, et al. A preclinical radiotherapy dosimetry audit using a realistic 3D printed murine phantom. Sci Rep. 2022;12(1):6826. https://doi.org/10.1038/s41598-022-10895-5 DOI: https://doi.org/10.1038/s41598-022-10895-5
Clausen M, Khachonkham S, Gruber S, et al. Phantom design and dosimetric characterization for multiple simultaneous cell irradiations with active pencil beam scanning. Radiat Environ Biophys. 2019;58(4):563–73. https://doi.org/10.1007/s00411-019-00813-1 DOI: https://doi.org/10.1007/s00411-019-00813-1
Poon C. Measuring the density and viscosity of culture media for optimized computational fluid dynamics analysis of in vitro devices. J Mech Behav Biomed Mater. 2022;126:105024. https://doi.org/10.1016/j.jmbbm.2021.105024 DOI: https://doi.org/10.1016/j.jmbbm.2021.105024
Brix N, Samaga D, Belka C, Zitzelsberger H, Lauber K. Analysis of clonogenic growth in vitro. Nat Protocols. 2021;16(11):4963–91. https://doi.org/10.1038/s41596-021-00615-0 DOI: https://doi.org/10.1038/s41596-021-00615-0
Jones L, Hoban P, Metcalfe P. The use of the linear quadratic model in radiotherapy: a review. Australas Phys Eng Sci Med. 2001;24(3):132–46. https://doi.org/10.1007/BF03178355 DOI: https://doi.org/10.1007/BF03178355
Yazdani S, Takabi F, Nickfarjam A. The commissioning and validation of eclipseTM treatment planning system on a Varian VitalBeamTM medical linear accelerator for Photon and electron beams. Front Biomed Technol. 2021;8. https://doi.org/10.18502/fbt.v8i2.6514 DOI: https://doi.org/10.18502/fbt.v8i2.6514
Dempsey JF, Low DA, Mutic S, et al. Validation of a precision radiochromic film dosimetry system for quantitative two-dimensional imaging of acute exposure dose distributions. Med Phys. 2000;27(10):2462–75. https://doi.org/10.1118/1.1290488[ DOI: https://doi.org/10.1118/1.1290488
Belli M, Cherubini R, Finotto S, et al. RBE-LET relationship for the survival of V79 cells irradiated with low energy protons. Int J Radiat Biol. 1989;55(1):93–104. https://doi.org/10.1080/09553008914550101 DOI: https://doi.org/10.1080/09553008914550101
Koch RA, Harmel C, Alber M, Bahn E. A framework for automated time-resolved analysis of cell colony growth after irradiation. Phys Med Biol. 2021;66(3):035017. https://doi.org/10.1088/1361-6560/abd00d DOI: https://doi.org/10.1088/1361-6560/abd00d
Additional Files
Published
How to Cite
Issue
Section
License
Copyright (c) 2024 Anders Tobias Frederiksen, Morten Bjørn Jensen, Per Rugaard Poulsen, Niels Bassler, Brita Singers Sørensen, Mateusz Sitarz
This work is licensed under a Creative Commons Attribution 4.0 International License.
