Frozen tissue biobanks. Tissue handling, cryopreservation, extraction, and use for proteomic analysis

Authors

  • Christer Ericsson Department of Oncology – Pathology, Karolinska Institutet, CCK R8:05, Karolinska University Hospital, Solna, 171 76, Stockholm, Sweden
  • Bo Franzén DMPK Research & Biomarkers, Local Discovery, Research Area CNS & Pain Control, AstraZeneca R&D Södertälje, SE-15185, Södertälje, Sweden
  • Monica Nistér Department of Oncology – Pathology, Karolinska Institutet, CCK R8:05, Karolinska University Hospital, Solna, 171 76, Stockholm, Sweden

DOI:

https://doi.org/10.1080/02841860600818047

Abstract

The potential and the limitations of protein analysis of tissue samples are surveyed. The complexity, concentration range and dynamics of the human proteome are reviewed, as is the effect of handling and cryopreservation. Protein extraction, solubilization, resolution and detection are discussed, in relation to the properties of the human proteome.

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Published

2006-01-01

How to Cite

Ericsson, C., Franzén, B., & Nistér, M. (2006). Frozen tissue biobanks. Tissue handling, cryopreservation, extraction, and use for proteomic analysis. Acta Oncologica, 45(6), 643–661. https://doi.org/10.1080/02841860600818047

Issue

Vol. 45 No. 6 (2006): Acta Oncologica

Section

Articles