Bone Cell Viability After Irradiation: An enzyme histochemical study

Authors

  • M. Jacobsson E.N.T.-Department Of Sahlgrenska Sjukhuset, The Laboratory Of Experimental Biology, Department Of Anatomy, And The Department Of Oncology And Radiotherapy, University of Gothenburg, Gothenburg, Sweden
  • P. Kälebo E.N.T.-Department Of Sahlgrenska Sjukhuset, The Laboratory Of Experimental Biology, Department Of Anatomy, And The Department Of Oncology And Radiotherapy, University of Gothenburg, Gothenburg, Sweden
  • A. Tjellström E.N.T.-Department Of Sahlgrenska Sjukhuset, The Laboratory Of Experimental Biology, Department Of Anatomy, And The Department Of Oncology And Radiotherapy, University of Gothenburg, Gothenburg, Sweden
  • I. Turesson E.N.T.-Department Of Sahlgrenska Sjukhuset, The Laboratory Of Experimental Biology, Department Of Anatomy, And The Department Of Oncology And Radiotherapy, University of Gothenburg, Gothenburg, Sweden

DOI:

https://doi.org/10.3109/02841868709113719

Keywords:

Radiobiology, cell studies, Rabbits, gamma-irradiation, osteocytes, diaphorase activity, viability

Abstract

Adult rabbits were irradiated to one proximal tibial metaphysis while the contralateral tibia served as a control. Each animal was thus its own control. Single doses of 15, 25 and 40 Gy 60Co were used. The follow-up time was 11 to 22 weeks after irradiation. A histochemical method, recording diaphorase (NADH2 and NADPH2) activity in osteocytes, was employed. This method is regarded as superior to conventional histology. No evidence of osteocyte death was found even after 22 weeks following 40 Gy irradiation. This is interpreted as an indication that the osteocytes, which are end stage cells, are relatively radioresistant.

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Published

1987-01-01

How to Cite

Jacobsson, M., Kälebo, P., Tjellström, A., & Turesson, I. (1987). Bone Cell Viability After Irradiation: An enzyme histochemical study. Acta Oncologica, 26(6), 463–465. https://doi.org/10.3109/02841868709113719

Issue

Vol. 26 No. 6 (1987): Acta Oncologica

Section

Articles