Differential Effect of Benzamide on Nad+ Content and the Frequency of Chromatid Aberrations in X-Irradiated L5178Y-R and L5178Y-S Cells

Authors

  • I. Szumiel From the Department of Radiobiology, Health Protection, Institute of Nuclear Chemistry and Technology, PL-03-195 Warsaw Poland the Department of Radioecology, the Swedish University of Agricultural Sciences, S-75007, Uppsala, Sweden
  • D. Wlodek From the Department of Radiobiology, Health Protection, Institute of Nuclear Chemistry and Technology, PL-03-195 Warsaw Poland the Department of Radioecology, the Swedish University of Agricultural Sciences, S-75007, Uppsala, Sweden
  • K. J. Johanson From the Department of Radiobiology, Health Protection, Institute of Nuclear Chemistry and Technology, PL-03-195 Warsaw Poland the Department of Radioecology, the Swedish University of Agricultural Sciences, S-75007, Uppsala, Sweden

DOI:

https://doi.org/10.3109/02841868809094371

Keywords:

Radiobiology, cell studies, x-irradiation, benzamide, NAD , chromatid aberrations, L5178Y cells

Abstract

Abstract

The effect of treatment with benzamide, an inhibitor of adenosine diphosphate (ADP) ribosyl polymerase (ADPRP) was studied in cells of two strains of L5178Y (LY) murine lymphoma exposed to ionizing radiation. Continuous 2 mmol/1 benzamide (Bz) treatment increased the frequency of chromatid aberrations in the radiation sensitive LY-S strain, but not in the resistant LY-R strain. This result is in agreement with the previously found, enhanced by Bz, killing effect of roentgen irradiation in LY-S cells. Also, the decrease in the cellular NAD+ content in these cells after irradiation was more pronounced than in LY-R cells; this may indicate an increased ADPRP activity upon infliction of DNA damage, or a difference in poly(ADP-ribose) turnover.

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Published

1988-01-01

How to Cite

Szumiel, I., Wlodek, D., & Johanson, K. J. (1988). Differential Effect of Benzamide on Nad+ Content and the Frequency of Chromatid Aberrations in X-Irradiated L5178Y-R and L5178Y-S Cells. Acta Oncologica, 27(6), 851–855. https://doi.org/10.3109/02841868809094371

Issue

Vol. 27 No. 6 (1988): Acta Oncologica

Section

Articles