The Expression of Glutathione Transferase Isoenzymes in Human Malignant Lymphoma Biopsies

Authors

  • Kiflu Berhane Department of Biochemistry, Uppsala University, Biomedical Center, Uppsala
  • Xiao-Yong Hao Department of Biochemistry, Uppsala University, Biomedical Center, Uppsala
  • Birger Christenson Department of Pathology, Karolinska Hospital, Stockholm, Sweden
  • Johan Hansson Department of Oncology, Radiumhemmet, Karolinska Hospital, Stockholm
  • Ulrik Ringborg Department of Oncology, Radiumhemmet, Karolinska Hospital, Stockholm
  • Bengt Mannervik Department of Biochemistry, Uppsala University, Biomedical Center, Uppsala

DOI:

https://doi.org/10.3109/02841869509093636

Abstract

Glutathione transferase (GST) activity as well as the expression of different classes of GST isoenzymes were studied in 14 lymphoma biopsies. The GST activity measured with 1-chloro-2,4-dinitrobenzene as a substrate, varied almost 9-fold. The expression of GSTs classes Pi, Alpha and Mu was studied by immunoblotting using antibodies against human GSTs. All lymphoma samples displayed high levels of class Pi GST. Class Alpha and Mu GSTs expression varied from not detectable to high. The observations were confirmed by quantitation of the three classes of GST with an ELISA technique. Nine of the patients were treated with bifunctional alkylating agents. A correlation between a clinical complete response to chemotherapy and low expression of GST Alpha was noted (p < 0.02).

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Published

1995-01-01

How to Cite

Berhane, K., Hao, X.-Y., Christenson, B., Hansson, J., Ringborg, U., & Mannervik, B. (1995). The Expression of Glutathione Transferase Isoenzymes in Human Malignant Lymphoma Biopsies. Acta Oncologica, 34(1), 35–41. https://doi.org/10.3109/02841869509093636

Issue

Vol. 34 No. 1 (1995): Acta Oncologica

Section

Articles