DNA Content in Non-Hodgkin's Lymphoma: Comparison between flow cytometry and cytogenetics in fresh and paraffin-embedded tissue

Authors

  • Rosa García Departments of Immunology, Fundacion Jiménez Díaz, Madrid, Spain
  • Ana Bueno Departments of Immunology, Fundacion Jiménez Díaz, Madrid, Spain
  • Esperanza Feijoó Departments of Immunology, Fundacion Jiménez Díaz, Madrid, Spain
  • Fernando Ortíz Departments of Immunology, Fundacion Jiménez Díaz, Madrid, Spain
  • Mercedes Robledo Departments of Genetics, Fundacion Jiménez Díaz, Madrid, Spain
  • Javier Benítez Departments of Genetics, Fundacion Jiménez Díaz, Madrid, Spain
  • Gemma Echezarreta Departments of Pathology, Fundacion Jiménez Díaz, Madrid, Spain
  • Jose Luis Sarasa Departments of Pathology, Fundacion Jiménez Díaz, Madrid, Spain
  • Carmen Rivas Departments of Pathology, Fundacion Jiménez Díaz, Madrid, Spain

DOI:

https://doi.org/10.3109/02841869409121772

Abstract

DNA content of 36-non-Hodgkin's lymphomas was analyzed by flow cytometry (FCM) and cytogenetics (CG), 21 in fresh and 15 in paraffin-embedded tissue. The results of both techniques were coincident in 60% of the fresh tissue samples and in 45% of the paraffin-embedded ones, the reason for this difference could be the poor resolution of DNA histograms from paraffin-embedded tissue. All samples judged as aneuploid by FCM were aneuploid also by CG. Some samples with a hyperdiploid population by CG gave a diploid population by FCM with a ‘false’ high DNA-synthesis (S) fraction. From a technical point of view, CG and FCM have to be performed on the same fresh tissue.

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Published

1994-01-01

How to Cite

García, R., Bueno, A., Feijoó, E., Ortíz, F., Robledo, M., Benítez, J., … Rivas, C. (1994). DNA Content in Non-Hodgkin’s Lymphoma: Comparison between flow cytometry and cytogenetics in fresh and paraffin-embedded tissue. Acta Oncologica, 33(6), 621–625. https://doi.org/10.3109/02841869409121772

Issue

Vol. 33 No. 6 (1994): Acta Oncologica

Section

Articles