Flow Cytometric Measurement of Rna Synthesis Based on Bromouridine Labelling and Combined with Measurement of Dna Content or Cell Surface Antigen

Authors

  • Peter Ø. Jensen Finsen Laboratory, Rigshospitalet, University Hospital, Copenhagen, Denmark
  • Jacob Larsen Finsen Laboratory, Rigshospitalet, University Hospital, Copenhagen, Denmark
  • Jørgen K. Larsen Finsen Laboratory, Rigshospitalet, University Hospital, Copenhagen, Denmark

DOI:

https://doi.org/10.3109/02841869309096111

Abstract

RNA synthesis can be analysed in nuclei or cells labelled with 5-bromouridine (BrUrd) and stained using cross-reacting anti-bromodeoxyuridine (BrdUrd) antibody. Flow cytometric dual parameter analysis of BrUrd incorporation and DNA content in nuclear suspensions of human blood lymphocytes showed that RNA synthesis increased within the first 24 hours of phytohemagglutinin (PHA) stimulation, reaching a maximum at 48 hours, when cells had entered the cell cycle. Using a new method for flow cytometric dual parameter analysis of BrUrd incorporation and a cell surface antigen, spontaneous RNA synthesis in HL-60 and K-562 cells was measured simultaneous with CD 13 expression.

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Published

1993-01-01

How to Cite

Jensen, P. Ø. ., Larsen, J. ., & Larsen, J. K. . (1993). Flow Cytometric Measurement of Rna Synthesis Based on Bromouridine Labelling and Combined with Measurement of Dna Content or Cell Surface Antigen. Acta Oncologica, 32(5), 521–524. https://doi.org/10.3109/02841869309096111

Issue

Vol. 32 No. 5 (1993): Acta Oncologica

Section

Articles