Ploidy Level and Tumor Progression in Prostatic Carcinoma

Abstract

Methods developed for cytophotometric analysis of archival tumor specimens used in retrospective studies were evaluated quantitatively. Up to 20-year-old May-Grünwald-Giemsa stained cytological slides could be Feulgen stained with excellent results. By expressing DNA data from the tumor cells as relative values (c-values) related to the internal staining control (mean value 2c) detailed ploidy level determinations could be made as accurately from measurements of old, destained slides as from measurements of cells from fresh tumor material, which were directly Feulgen-stained. Ploidy level determinations of prostatic carcinoma in 213 patients