Free Radicals as Potential Mediators of Metal Allergy: Effect of ascorbic Acid on Lymphocyte Proliferation and IFN-Gamma Production in Contact Allergy to Ni2+ and Co2+.
DOI:
https://doi.org/10.1080/000155598433395Abstract
A possible free radical mechanism in metal allergy was investigated in peripheral blood mononuclear cell (PBMC) cultures from 6 subjects;contact allergic to Ni2+ and Co2+;and 6 control individuals. Ni2+ and Co(2+)-mediated free radical generation was studied with electron spin resonance spectroscopy. The immune response was characterized by cellular [methyl-3H]thymidine uptake and interferon-gamma (IFN-gamma) production Ni2+ and Co2+ (10-50 microM) significantly increased lymphocyte proliferation and IFN-gamma production in PBMC cultures from contact allergic subjects in comparison with cultures from controls. Inhibition of Co(2+)-mediated free radical generation by ascorbic acid did not influence cellular [methyl-3H]thymidine uptake and IFN production. Detectable amounts of free radicals were not obtained with Ni2+. We therefore conclude that it is unlikely that free radicals are involved in contact allergy to Ni2+ and Co2+.Downloads
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Published
1998-04-16
How to Cite
Van Den Broeke, L., Gräslund, A., Larsson, P., Nilsson, J., Wahlberg, J., Scheynius, A., & Karlberg, A. (1998). Free Radicals as Potential Mediators of Metal Allergy: Effect of ascorbic Acid on Lymphocyte Proliferation and IFN-Gamma Production in Contact Allergy to Ni2+ and Co2+. Acta Dermato-Venereologica, 78(2), 95–98. https://doi.org/10.1080/000155598433395
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