Cryosectioning of hair follicles. An improved method using liquid nitrogen conduction freezing.
DOI:
https://doi.org/10.2340/0001555570335338Abstract
Horizontal sectioning of scalp biopsies is especially useful in hair diseases characterized by a reduction of follicles in size or number. Horizontal sectioning using standard cryo-microtomes is hampered by the differences in cutting properties between hair follicles and fatty tissue, resulting in loss of topography. We report a simple, inexpensive method to temporarily cool the specimen to an appropriate temperature by means of a conduction system using liquid nitrogen. Immunohistological methods such as immunofluorescence and immunoperoxidase techniques can be applied without restrictions.Downloads
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Published
1990-07-01
How to Cite
Van Baar, H. M., Schalkwijk, J., & Perret, C. M. (1990). Cryosectioning of hair follicles. An improved method using liquid nitrogen conduction freezing. Acta Dermato-Venereologica, 70(4), 335–338. https://doi.org/10.2340/0001555570335338
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