Binding specificity of rabbit anti-guinea pig epidermal cell sera: comparison of their receptors with those of concanavalin A and pemphigus sera

Abstract

Rabbit anti-guinea pig epidermal cell sera (AES) which were prepared by immunizing rabbits with enzymatically dispersed viable guinea pig epidermal cells were shown to react to the intercellular substances of stratified squamous epithelia of guinea pigs and monkeys in a pattern similar to that seen with concanavalin A (ConA) or pemphigus sera (PS) by immunofluorescence. Reciprocal blocking tests were performed on guinea pig lip mucosa after removal of the non-specific binding substances between preincubated substances and subsequently incubated ones. No definite inhibition was noted in the subsequent reactions upon preincubation with AES or PS. However, preincubation with ConA weakened the subsequent reaction with PS, but did not block the reaction with AES. Effects of solvents on the receptors for AES, ConA and PS were also examined. ConA receptor was resistant to both saline solution and ethanol (95% and 99%). PS receptor was labile to both saline solution and ethanol, while AES receptor was labile to ethanol, but resistant to saline solution. These observations suggest that the receptor for AES differs from that for ConA or PS.