Detection of Circulating Tumour DNA in Plasma during the Treatment of In-transit Melanoma Metastases with Topical Imiquimod

Authors

  • Miguel Mansilla-Polo Dermatology Department, Hospital Universitario y Politécnico La Fe, Valencia, Spain; Instituto de Investigación Sanitaria La Fe (IIS La Fe), Valencia, Spain https://orcid.org/0000-0001-6730-6723
  • Blanca de Unamuno-Bustos Dermatology Department, Hospital Universitario y Politécnico La Fe, Valencia, Spain; Instituto de Investigación Sanitaria La Fe (IIS La Fe), Valencia, Spain https://orcid.org/0000-0003-4073-145X
  • Fernando Navarro-Blanco Dermatology Department, Hospital Universitario y Politécnico La Fe, Valencia, Spain; Instituto de Investigación Sanitaria La Fe (IIS La Fe), Valencia, Spain
  • Gema Pérez-Simó Molecular Biology Laboratory, Service of Clinical Analysis, Hospital Universitario y Politécnico La Fe, Valencia, Spain
  • Sarai Palanca-Suela Molecular Biology Laboratory, Service of Clinical Analysis, Hospital Universitario y Politécnico La Fe, Valencia, Spain
  • Rafael Botella-Estrada Dermatology Department, Hospital Universitario y Politécnico La Fe, Valencia, Spain; Instituto de Investigación Sanitaria La Fe (IIS La Fe), Valencia, Spain; Faculty of Medicine, Universidad de Valencia, Valencia, Spain

DOI:

https://doi.org/10.2340/actadv.v106.adv-2025-0229

Keywords:

Melanoma, Circulating Tumor DNA, Imiquimod, Skin Neoplasms

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References

Martínez-Vila C, Teixido C, Aya F, Martín R, González-Navarro EA, Alos L, et al. Detection of circulating tumor DNA in liquid biopsy: current techniques and potential applications in melanoma. Int J Mol Sci 2025; 26: 861. DOI: https://doi.org/10.3390/ijms26020861

Genta S, Araujo DV, Hueniken K, Pipinikas C, Ventura R, Rojas P, et al. Bespoke ctDNA for longitudinal detection of molecular residual disease in high-risk melanoma patients. ESMO Open 2024; 9: 103978. DOI: https://doi.org/10.1016/j.esmoop.2024.103978

Slusher N, Jones N, Nonaka T. Liquid biopsy for diagnostic and prognostic evaluation of melanoma. Front Cell Dev Biol 2024; 12: 1420360. DOI: https://doi.org/10.3389/fcell.2024.1420360

Lucci A, Addanki S, Chiang YJ, Meas S, Sarli VN, Upshaw JR, et al. Presence of circulating tumor cells predates imaging detection of relapse in patients with stage III melanoma. Cancers 2023; 15: 3630. DOI: https://doi.org/10.3390/cancers15143630

Marchisio S, Ricci AA, Roccuzzo G, Bongiovanni E, Ortolan E, Bertero L, et al. Monitoring circulating tumor DNA liquid biopsy in stage III BRAF-mutant melanoma patients undergoing adjuvant treatment. J Transl Med 2024; 22: 1074. DOI: https://doi.org/10.1186/s12967-024-05783-7

Palacios-Diaz RD, de Unamuno-Bustos B, Carratalá-García A, Pérez-Simó G, Moreno-Ramírez D, Ferrándiz L, et al. Circulating tumor DNA predicts tumor progression and poor survival in patients with stage III melanoma. Melanoma Res 2025; 35: 259–267. DOI: https://doi.org/10.1097/CMR.0000000000001041

Scarfì F, Patrizi A, Veronesi G, Lambertini M, Tartari F, Mussi M, et al. The role of topical imiquimod in melanoma cutaneous metastases: a critical review of the literature. Dermatol Ther 2020; 33: e14165. DOI: https://doi.org/10.1111/dth.14165

Kumar AB, Jakub JW, Lester SC, Markovic SN, Baum CL. In-transit melanoma metastases: evaluation and management for the dermatologist. J Am Acad Dermatol 2025; 93: 188–195. DOI: https://doi.org/10.1016/j.jaad.2025.03.005

El-Khattouti A, Selimovic D, Hannig M, Taylor EB, Abd Elmageed ZY, Hassan SY, et al. Imiquimod-induced apoptosis of melanoma cells is mediated by ER stress-dependent Noxa induction and enhanced by NF-κB inhibition. J Cell Mol Med 2016; 20: 266–286. DOI: https://doi.org/10.1111/jcmm.12718

Di Nardo L, Del Regno L, Di Stefani A, Mannino M, Fossati B, Catapano S, et al. The dynamics of circulating tumour DNA (ctDNA) during treatment reflects tumour response in advanced melanoma patients. Exp Dermatol 2023; 32: 1785–1793. DOI: https://doi.org/10.1111/exd.14901

Published

2026-06-16

How to Cite

Mansilla-Polo, M., Unamuno-Bustos, B. de, Navarro-Blanco, F., Pérez-Simó, G., Palanca-Suela, S., & Botella-Estrada, R. (2026). Detection of Circulating Tumour DNA in Plasma during the Treatment of In-transit Melanoma Metastases with Topical Imiquimod. Acta Dermato-Venereologica, 106, adv–2025. https://doi.org/10.2340/actadv.v106.adv-2025-0229

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