The Potential of Using Enzyme-linked Immunospot to Diagnose Cephalosporin-induced Maculopapular Exanthems

Authors

  • Boonthorn Tanvarasethee
  • Supranee Buranapraditkun
  • Jettanong Klaewsongkram

DOI:

https://doi.org/10.2340/00015555-1386

Keywords:

drug hypersensitivity, adverse drug reaction, ELISPOT.

Abstract

There is no reliable test to diagnose cephalosporin-induced maculopapular exanthems (MPE). This study aimed to evaluate the role of enzyme-linked immunospot assay in the diagnosis of cephalosporin-induced MPE compared with skin testing. A total of 25 patients with a history of cephalosporin-induced MPE were skin tested and the frequencies of cephalosporin-specific interferon-γ-, interleukin-5-, and interleukin-10-releasing cells/10(6) peripheral blood mononuclear cells were measured after stimulating with the culprit drug, compared with 20 non-allergic controls. Values greater than means+2 standard deviations of the values in non-allergic controls were considered diagnostic. The study showed that the combination of interferon-γ and interleukin-5 enzyme-linked immunospot assays was more sensitive than skin testing to diagnose cephalosporin allergy (40% vs. 8%, p = 0.008) and sensitivity increased to 57.1% when the test was performed within 2 years of the drug reaction. Enzyme-linked immunospot assay is a promising tool for confirming the diagnosis of cephalosporin-induced MPE.

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Published

2012-05-23

How to Cite

Tanvarasethee, B., Buranapraditkun, S., & Klaewsongkram, J. (2012). The Potential of Using Enzyme-linked Immunospot to Diagnose Cephalosporin-induced Maculopapular Exanthems. Acta Dermato-Venereologica, 93(1), 66–69. https://doi.org/10.2340/00015555-1386

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Section

Articles