Identification of a Major Yolk Protein as an Allergen in Sea Urchin Roe

Authors

  • Ayako Yamasaki
  • Hiromi Higaki
  • Keiko Nakashima
  • Osamu Yamamoto
  • Kyaw Zaw Hein
  • Hitoshi Takahashi
  • Yuko Chinuki
  • Eishin Morita

DOI:

https://doi.org/10.2340/00015555-0783

Keywords:

sea urchin roe, anaphylaxis, major yolk protein, allergen

Abstract

Anaphylaxis after eating sea urchin roe has been reported. However, its major allergens have not yet been identified. The aim of this study was to identify the major allergens of sea urchin roe. Proteins of sea urchin roe were separated by sodium dodecyl sulphate-polyacrylamide gel electrophoresis and two-dimensional electrophoresis (2-DE). An immunoglobulin (Ig)E-binding protein was detected by immunoblotting using the patient serum. An allergen isolated from 2DE-gel was identified by peptide mass fingerprinting using matrix-assisted laser desorption/ionization-time of flight-mass spectrometry. Immunoblot analysis of sea urchin extracts showed that a 160-kDa protein at pI 6–7 was recognized by the patient IgE. Peptide mass fingerprint analysis revealed that the protein was the major yolk protein (152 kDa, pI 6.9) of sea urchins. The results show that a major allergen of sea urchin roe is the major yolk protein.

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Published

2010-03-08

How to Cite

Yamasaki, A., Higaki, H., Nakashima, K., Yamamoto, O., Zaw Hein, K., Takahashi, H., Chinuki, Y., & Morita, E. (2010). Identification of a Major Yolk Protein as an Allergen in Sea Urchin Roe. Acta Dermato-Venereologica, 90(3), 235–238. https://doi.org/10.2340/00015555-0783

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Section

Articles