Low-dose Oral Methotrexate Induces Apoptosis of Tissue Eosinophils in Bullous Pemphigoid
DOI:
https://doi.org/10.2340/00015555-0423Keywords:
apoptosis, skin eosinophils, bullous pemphigoid, methotrexate.Abstract
We have shown previously that eosinophils are abundant in blood and tissue in bullous pemphigoid and that they rapidly disappear, without sign of necrosis, during methotrexate therapy. The aim of the present study was to investigate the role of apoptosis in this phenomenon. The terminal deoxynucleotidyl transferase-mediated dUTP-biotin nick-end labelling method for detecting apoptosis was used, combined with immunohistochemical detection of eosinophils before and during oral methotrexate. The numbers of apoptotic eosinophils were compared using light microscopy. Eosinophil apoptosis was significantly higher in skin after one week of methotrexate therapy and the number of eosinophils was reduced. The results indicate that methotrexate therapy induces apoptosis of the tissue eosinophils in bullous pemphigoid.Downloads
Downloads
Published
How to Cite
Issue
Section
License
Copyright (c) -1 Kristina Dahlman-Ghozlan, Jean-Paul Ortonne, Philippe Bahadoran, Anne Spadafora, Eija Stephansson
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
All digitalized ActaDV contents is available freely online. The Society for Publication of Acta Dermato-Venereologica owns the copyright for all material published until volume 88 (2008) and as from volume 89 (2009) the journal has been published fully Open Access, meaning the authors retain copyright to their work.
Unless otherwise specified, all Open Access articles are published under CC-BY-NC licences, allowing third parties to copy and redistribute the material in any medium or format and to remix, transform, and build upon the material for non-commercial purposes, provided proper attribution to the original work.
