Effect of Porphyromonas gingivalis infection in the placenta and umbilical cord in pregnant mice with low birth weight

Authors

  • Sayuri Udagawa Department of Periodontology, Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental University, Tokyo, Japan
  • Sayaka Katagiri Department of Periodontology, Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental University, Tokyo, Japan
  • Shogo Maekawa Department of Periodontology, Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental University, Tokyo, Japan
  • Yasuo Takeuchi Department of Periodontology, Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental University, Tokyo, Japan
  • Rina Komazaki Department of Periodontology, Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental University, Tokyo, Japan
  • Anri Ohtsu Department of Periodontology, Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental University, Tokyo, Japan
  • Naoki Sasaki Department of Periodontology, Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental University, Tokyo, Japan
  • Takahiko Shiba Department of Periodontology, Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental University, Tokyo, Japan
  • Kazuki Watanabe Department of Periodontology, Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental University, Tokyo, Japan
  • Kazuyuki Ishihara Oral Health Science Center, Tokyo Dental College, Tokyo, Japan; Department of Microbiology, Tokyo Dental College, Tokyo, Japan
  • Noriko Sato Department of Molecular Epidemiology, Medical Research Institute, Tokyo Medical and Dental University, Tokyo, Japan
  • Naoyuki Miyasaka Department of Comprehensive Reproductive Medicine, Tokyo Medical and Dental University, Tokyo, Japan
  • Yuichi Izumi Department of Periodontology, Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental University, Tokyo, Japan

DOI:

https://doi.org/10.1080/00016357.2018.1426876

Keywords:

Periodontitis, low birth weight, Porphyromonas gingivalis, placenta, umbilical cord

Abstract

Objective: Growing evidence indicates an association between periodontitis and delivery outcome; however, the mechanism is unclear. This study aimed to investigate the influence of Porphyromonas gingivalis (Pg) infection on delivery outcome in mice.

Materials and methods: Bacteremia was induced in pregnant Slc:ICR mice (8 weeks old) by intravenous injection of Pg. Mice were randomly divided into a control group (CO), and those receiving Pg injection at gestational day 1 (GD1), gestational day 15 (GD15) or every day (ED). Delivery outcome, Pg infection, and gene expression in the placenta and umbilical cord were evaluated.

Results: Birth weight was lower in the ED and GD15 groups than in the CO group. A remarkable increase in anti-Pg IgG antibody was observed in the ED and GD1 groups, although Pg was not detected in the placenta or umbilical cord. mRNA expression of Tnfα and Il6 in the placenta, and Hif1α in the umbilical cord, was significantly increased in the ED group. Microarray analysis of the umbilical cord revealed increased expression of several genes including Orm1, Mgl2, Rps6ka3 and Trim15 in the ED group.

Conclusions: Pg infection during the third trimester caused low birth weight and inflammation in the placenta and umbilical cord.

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Published

2018-08-18

Issue

Vol. 76 No. 6 (2018): Acta Odontologica Scandinavica

Section

Articles