Cathepsin D: Purification from rat liver and immunohistochemical demonstration in rat incisor

Authors

  • A. Linde Laboratory of Oral Biology, Department of Histology, University of Gothenburg, Sweden
  • B. Persliden Laboratory of Oral Biology, Department of Histology, University of Gothenburg, Sweden
  • L. Röunnbäck Laboratory of Oral Biology, Department of Histology, University of Gothenburg, Sweden

DOI:

https://doi.org/10.3109/00016357809004660

Abstract

Rat liver cathepsin D (EC 3.4.23.5) was purified using precipitation technique, ion exchange chromatography, molecular sieve chiomatography and isoelectric focusing. Rabbit anti-rat cathepsin D IgG was prepared and rat incisor teeth were cross-sectioned in a cryostat. These sections were incubated with FITC-conjugated anti-rat cathepsin D IgG. Marked fluorescence, indicating the localization of cathepsin D. could be seen over the odontoblast and predentin area. No specific fluorescence could be demonstrated in the pulp connective tissue proper nor in the dentin

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Published

1978-01-01

Issue

Vol. 36 No. 3 (1978): Acta Odontologica Scandinavica

Section

Articles