Single nucleotide polymorphism rs4284505 in microRNA17 and risk of dental fluorosis

Authors

  • Zerrin Abbasoglu Department of Pediatric Dentistry, Yeditepe University, Istanbul, Turkey
  • Mariana Dalledone School of Health and Biological Sciences, Universidade Positivo, Curitiba, Brazil
  • Letícia M. Wambier Department of Dentistry, School of Health and Biological Sciences, Universidade Positivo, Curitiba, Brazil
  • Giovana Pecharki Department of Community Health, Federal University of Parana, Curitiba, Brazil
  • Flares Baratto-Filho School of Health and Biological Sciences, Universidade Positivo, Curitiba, Brazil;School of Dentistry, Univille University, Joinville, Brazil
  • Kesly Mary R. Andrades School of Dentistry, Univille University, Joinville, Brazil
  • Rafaela Scariot School of Health and Biological Sciences, Universidade Positivo, Curitiba, Brazil
  • Paula C. Trevilatto Dental School, Pontifícia Universidade Católica do Paraná (PUC-PR), Curitiba, Brazil
  • João A. Brancher School of Health and Biological Sciences, Universidade Positivo, Curitiba, Brazil
  • Erika C. Küchler School of Health and Biological Sciences, Universidade Positivo, Curitiba, Brazil;School of Dentistry, Univille University, Joinville, Brazil

DOI:

https://doi.org/10.1080/00016357.2020.1786600

Keywords:

microRNA, oestrogen receptor alpha gene, polymorphism, dental fluorosis

Abstract

Objectives

The aim of this study is to evaluate the association between the single nucleotide polymorphism (SNP) rs4284505 within the gene that codifies microRNA17 (miRNA17) and dental fluorosis (DF) in a group of children.

Methods

Children living in a city with fluoridation of public water supplies were included. DF was assessed in erupted permanent teeth by Dean’s modified index. The miR-SNP rs4284505 was selected in miRNA17 and genotyping was carried out by real-time PCR. Genotype and allelic distributions between DF and control, and between DF phenotypes (mild, moderate and severe) and control were analysed.

Results

Among a total of 527 children enrolled for the study, 383 were DF free and 144 presented DF. In the dominant model analysis (AA + AG vs. GG) the miR-SNP rs4284505 was associated with moderate DF, with carriers of the GG genotype having an increased risk of more than two times for DF (p = 0.031; Odds Ratio = 2.26, Confidence Interval 95%= 1.04-4.73). Allelic distribution showed borderline statistical significance for moderate DF with the carriers of G allele having an increased risk for DF (p = .050; Odds Ratio = 1.75, Confidence Interval 95%= 1.00–3.12).

Conclusion

The miR-SNP rs4284505 in miRNA17 was associated with an increased risk of DF.

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Published

2020-08-17

Issue

Vol. 78 No. 6 (2020): Acta Odontologica Scandinavica

Section

Articles