Impact of resveratrol in the reduction of the harmful effect of diabetes on peri-implant bone repair: bone-related gene expression, counter-torque and micro-CT analysis in rats

Authors

  • Monica Grazieli Corrêa a Dental Research Division, School of Dentistry, Paulista University, São Paulo, Brazil
  • Fernanda Vieira Ribeiro a Dental Research Division, School of Dentistry, Paulista University, São Paulo, Brazil
  • Suzana Peres Pimentel a Dental Research Division, School of Dentistry, Paulista University, São Paulo, Brazil
  • Bruno Braga Benatti b School of Dentistry, Federal University of Maranhão, São Luís, Brazil
  • Pedro Henrique Felix Silva c Department of Surgery and Bucco-Maxillofacial Traumatology and Periodontology, Ribeirão Preto School of Dentistry, University of São Paulo, Ribeirão Preto, Brazil
  • Marcio Zaffalon Casati a Dental Research Division, School of Dentistry, Paulista University, São Paulo, Brazil
  • Fabiano Ribeiro Cirano a Dental Research Division, School of Dentistry, Paulista University, São Paulo, Brazil

DOI:

https://doi.org/10.1080/00016357.2020.1797159

Keywords:

Diabetes mellitus, dental implants, gene expression, osseointegration, resveratrol

Abstract

Objective

Investigate the impact of resveratrol (RESV) on peri-implant repair and its effect on bone-related markers in rats with induced diabetes mellitus (DM).

Material and methods

Ninety rats were divided into: DM + RESV (n = 18); DM + placebo (PLAC) (n = 18); DM + insulin (INS) (n = 18); DM + RESV + INS (n = 18); Non-DM (n = 18). Diabetes was induced by streptozotocin. One screw-shaped titanium implant was inserted in each tibiae of animals. Treatments were administered during 30 days. After, one of the implants was removed for counter-torque and the peri-implant tissue was collected for mRNA quantification of BMP-2, OPN, Runx2, Lrp-5, Osx, β-catenin, Dkk1, OPG, and RANKL by Real-time PCR. The other tibia was submitted to MicroCT analysis to measure: bone volume (BV/TV), trabecular thickness (Tb.Th) and bone-implant contact (BIC).

Results

Higher counter-torque values were observed for implant removal in DM + RESV, DM + RESV + INS and Non-DM groups when compared to DM + PLAC (p < .05). Augmented Tb.Th was observed in DM + RESV and Non-DM when compared to DM + PLAC group (p < .05), whereas higher BIC was detected in DM + RESV, DM + RESV + INS and Non-DM animals when compared to DM + PLAC (p < .05). Levels of RANKL were downregulated by the RESV and/or INS therapy, whereas only the association of RESV and INS upregulated the levels of Runx2 (p < .05).

Conclusions

The therapy with RESV may favour peri-implant bone repair improving bone formation around implants.

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Published

2021-03-04

Issue

Vol. 79 No. 3 (2021): Acta Odontologica Scandinavica

Section

Articles