TMT-labelled quantitative proteomic analysis to predict the target promoting human odontogenic inflammatory granulation tissue transform into reparative granulation tissue

Authors

  • Jianying Zhang a Department of Oral and Maxillofacial Surgery, School of Stomatology, State Key Laboratory of Military Stomatology & National Clinical Research Center for Oral Diseases & Shaanxi Clinical Research Center for Oral Diseases, The Fourth Military Medical University, Xi'an, China
  • Dengke Li a Department of Oral and Maxillofacial Surgery, School of Stomatology, State Key Laboratory of Military Stomatology & National Clinical Research Center for Oral Diseases & Shaanxi Clinical Research Center for Oral Diseases, The Fourth Military Medical University, Xi'an, China
  • Xueni Zheng a Department of Oral and Maxillofacial Surgery, School of Stomatology, State Key Laboratory of Military Stomatology & National Clinical Research Center for Oral Diseases & Shaanxi Clinical Research Center for Oral Diseases, The Fourth Military Medical University, Xi'an, China
  • Wuyang Zhang a Department of Oral and Maxillofacial Surgery, School of Stomatology, State Key Laboratory of Military Stomatology & National Clinical Research Center for Oral Diseases & Shaanxi Clinical Research Center for Oral Diseases, The Fourth Military Medical University, Xi'an, China
  • Rui Hou a Department of Oral and Maxillofacial Surgery, School of Stomatology, State Key Laboratory of Military Stomatology & National Clinical Research Center for Oral Diseases & Shaanxi Clinical Research Center for Oral Diseases, The Fourth Military Medical University, Xi'an, China
  • Changkui Liu b Department of Oral and Maxillofacial Surgery, School of Stomatology, Xi'an Medical University, Xi'an, China
  • Yu Zhang a Department of Oral and Maxillofacial Surgery, School of Stomatology, State Key Laboratory of Military Stomatology & National Clinical Research Center for Oral Diseases & Shaanxi Clinical Research Center for Oral Diseases, The Fourth Military Medical University, Xi'an, China
  • Kaijin Hu a Department of Oral and Maxillofacial Surgery, School of Stomatology, State Key Laboratory of Military Stomatology & National Clinical Research Center for Oral Diseases & Shaanxi Clinical Research Center for Oral Diseases, The Fourth Military Medical University, Xi'an, China
  • Hongzhi Zhou a Department of Oral and Maxillofacial Surgery, School of Stomatology, State Key Laboratory of Military Stomatology & National Clinical Research Center for Oral Diseases & Shaanxi Clinical Research Center for Oral Diseases, The Fourth Military Medical University, Xi'an, China
  • Yang Xue a Department of Oral and Maxillofacial Surgery, School of Stomatology, State Key Laboratory of Military Stomatology & National Clinical Research Center for Oral Diseases & Shaanxi Clinical Research Center for Oral Diseases, The Fourth Military Medical University, Xi'an, China

DOI:

https://doi.org/10.1080/00016357.2021.1890817

Keywords:

Odontogenic inflammatory diseases, granulation tissue, proteomic, extracellular matrix, cathepsin K (CTSK)

Abstract

 

Objectives

Odontogenic inflammatory diseases are main causes for alveolar bone breakdown and teeth loss, leaving great difficulties in denture restoration. Local inflammatory granulation tissue (IGT) is considered as pathological tissue and required to be removed. However, there are many evidences supporting that under appropriate intervention, IGT in alveolar bone maybe transformed into reparative granulation tissue (RGT), followed by ossification. Therefore, this study aimed to discover a specific target to promote this transformation.

Materials and methods

After drawing out histological differences between IGT and RGT with haematoxylin and eosin (H&E) and immunohistochemical (IHC) assay staining, TMT-labelled quantitative proteomic analysis was applied to identify potential targets.

Results

The most striking histological property of RGT was found to be ECM deposition, which significantly decreased inflammatory cells, prominently increased fibroblasts as well as triggered changes of vascular types. Combined with histological findings and proteomic analysis, five KEGG pathways were associated with ECM, inflammation and angiogenesis and 49 pathways involved in differentially expressed proteins. COL1A1 was not only the most up-regulated protein, but also one of main hubs in protein–protein interaction regulatory network. Specific protease cathepsin K (CTSK) was identified. Level of CTSK in RGT was down-regulated to 69.10–76.97% (p < .05), with significantly up-regulated COL1A1, COL1A2, FN1 and TGFB1 included in focal adhesion, PI3K-Akt signalling pathways and angiogenesis. CTSK involved in transformation from IGT to RGT.

Conclusions

CTSK might be a target to regulate transformation from IGT to RGT in alveolar bone through ECM, stem cells and angiogenesis mechanisms. However, further research is also clearly required.

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Published

2021-08-18

Issue

Vol. 79 No. 6 (2021): Acta Odontologica Scandinavica

Section

Articles