Tumour necrosis factor-alpha polymorphism -308 G/a and its protein in subjects with gingivitis

Authors

  • Julieta Sarai Becerra-Ruiz a Los Altos University Center, University of Guadalajara, Tepatitlán de Morelos, México
  • Saúl Ramírez-De los Santos b Biosciences Research Institute, Los Altos University Center, University of Guadalajara, Tepatitlán de Morelos, México
  • Carmen Celina Alonso-Sánchez a Los Altos University Center, University of Guadalajara, Tepatitlán de Morelos, México
  • Fernando Martínez-Esquivias a Los Altos University Center, University of Guadalajara, Tepatitlán de Morelos, México
  • Luz Andrea Martínez-Pérez a Los Altos University Center, University of Guadalajara, Tepatitlán de Morelos, México
  • Ana Carolina Padilla-González c Department of Clinics, Los Altos University Center, University of Guadalajara, Tepatitlán de Morelos, México
  • Gregorio Alejandro Rivera-Santana c Department of Clinics, Los Altos University Center, University of Guadalajara, Tepatitlán de Morelos, México
  • Celia Guerrero- Velázquez d Institute for Research in Dentistry, University Center for Health Sciences, University of Guadalajara, Guadalajara, México
  • Edgar Iván López-Pulido b Biosciences Research Institute, Los Altos University Center, University of Guadalajara, Tepatitlán de Morelos, México
  • Juan Manuel Guzmán-Flores b Biosciences Research Institute, Los Altos University Center, University of Guadalajara, Tepatitlán de Morelos, México

DOI:

https://doi.org/10.1080/00016357.2021.1928280

Keywords:

TNF-alpha, polymorphism, periodontal disease, cytokine, salivary levels

Abstract

Objective

This study examined the association between tumour necrosis factor-alpha (TNF- α) (–308 G/A) polymorphism and gingivitis, and serum and salivary TNF- α levels, in a Mexican population.

Material and methods

This study enrolled 171 subjects, divided into two groups: healthy subjects and gingivitis patients. TNF- α (–308 G/A) gene polymorphism was analyzed by PCR-RFLP assay. Salivary and serum samples were used to measure cytokine levels through the ELISA technique.

Results

TNF- α (–308 G/A) polymorphism was shown to have a protective effect in carriers of the A/A genotype and allele A. The G/A genotype is associated with an increase in high-density lipoprotein cholesterol (HDL-C) levels in the gingivitis group. Healthy individuals had higher levels of salivary TNF- α and HDL-C, and increased salivary flow. Triglycerides, low-density lipoprotein cholesterol, and very low-density lipoprotein cholesterol levels were increased in the gingivitis group. No statistical differences were found in serum TNF- α levels.

Conclusion

Our data demonstrate that the TNF- α −308 A/A genotype exerts a protective effect against gingivitis. Moreover, oral conditions are associated with some biochemical parameters.

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Published

2021-11-17

Issue

Vol. 79 No. 8 (2021): Acta Odontologica Scandinavica

Section

Articles