Experimental model of periodontitis and rheumatoid arthritis potentiates the deleterious effects on functional capacity, leukocyte migration, synovial and periodontal tissues in Wistar rats

Authors

  • Thaís Caroline Schnaufer a Master’s of Science in Health and Bioscences, State University of Western Paraná - UNIOESTE, Cascavel, Brazil
  • Alana Ludemila Tavares a Master’s of Science in Health and Bioscences, State University of Western Paraná - UNIOESTE, Cascavel, Brazil
  • Iranilda Moha Hoss a Master’s of Science in Health and Bioscences, State University of Western Paraná - UNIOESTE, Cascavel, Brazil
  • Bruna Rafaela Correia a Master’s of Science in Health and Bioscences, State University of Western Paraná - UNIOESTE, Cascavel, Brazil
  • Lilian de Araújo Pradal a Master’s of Science in Health and Bioscences, State University of Western Paraná - UNIOESTE, Cascavel, Brazil
  • Ediana Amanda Piana b Department of Periodontology, State University of Western Paraná - UNIOESTE, Cascavel, Brazil
  • Franciane Wachter b Department of Periodontology, State University of Western Paraná - UNIOESTE, Cascavel, Brazil
  • Taciane Stein c Health and Bioscences, State University of Western Paraná - UNIOESTE, Cascavel, Brazil
  • Thais Soprani Ayala d Center of Medical and Pharmaceutical Sciences, State University of Western Paraná - UNIOESTE, Cascavel, Brazil
  • Rafael Andrade Menolli d Center of Medical and Pharmaceutical Sciences, State University of Western Paraná - UNIOESTE, Cascavel, Brazil
  • Gladson Ricardo Flor Bertolini e Center of Biological and Health Sciences, State University of Western Paraná - UNIOESTE, Cascavel, Brazil
  • Rose Meire Costa e Center of Biological and Health Sciences, State University of Western Paraná - UNIOESTE, Cascavel, Brazil
  • Lucinéia de Fátima Chasko Ribeiro e Center of Biological and Health Sciences, State University of Western Paraná - UNIOESTE, Cascavel, Brazil
  • Carlos Augusto Nassar b Department of Periodontology, State University of Western Paraná - UNIOESTE, Cascavel, Brazil
  • Patrícia Oehlmeyer Nassar b Department of Periodontology, State University of Western Paraná - UNIOESTE, Cascavel, Brazil

DOI:

https://doi.org/10.1080/00016357.2022.2078504

Keywords:

Ligature, experimental arthritis, inflammation, Freund’s adjuvant

Abstract

Objective

This study aimed to evaluate whether ligature-induced periodontitis and rheumatoid arthritis (RA) potentiate the deleterious effects on functional capacity, periodontal and synovial tissues, leukocyte migration, and interleukin 17 (IL-17) levels, and to investigate the repercussions of single Freund’s Complete Adjuvant (FCA) injection associated with periodontitis.

Materials and methods

Fifty-one male Wistar rats were randomised into six groups: control (CG, n = 8), RA (RAG, n = 9), periodontitis (PG, n = 9), periodontitis and RA (PRAG, n = 9), periodontitis and intradermal injection (PIDG, n = 9), and periodontitis and intra-articular injection (PIAG, n = 7). The animals underwent ligature placement and one or two injections with FCA to induce RA. Motor disability, nociceptive threshold, joint edema, and muscle strength were assessed, and the animals were euthanized on day 30. Synovial fluid, hemimandibles, and knee joints were collected.

Results

PRAG showed no reduction of edema or improvement of muscle strength, whereas it showed most significant changes in leukocyte migration, morphological analyses of the synovial membrane (SM), and radiographic and histometric analyses of the jaw. The PIAG showed some alterations, though not permanent.

Conclusion

Ligature-induced periodontitis and RA induced by two FCA injections accentuated the deleterious effects on functional capacity, leukocyte migration, synovial and periodontal tissues.

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Published

2023-01-02

Issue

Vol. 81 No. 1 (2023): Acta Odontologica Scandinavica

Section

Articles