Intratubular disinfection with tri-antibiotic and calcium hydroxide pastes

Authors

  • Thais Cristina Pereira Department of Dentistry, Endodontics and Dental Materials, Bauru Dental School, University of São Paulo, Bauru, SP, Brazil
  • Layla Reginna Silva Munhoz De Vasconcelos Department of Dentistry, Endodontics and Dental Materials, Bauru Dental School, University of São Paulo, Bauru, SP, Brazil
  • Marcia Sirlene Zardin Graeff Department of Dentistry, Endodontics and Dental Materials, Bauru Dental School, University of São Paulo, Bauru, SP, Brazil
  • Marco Antonio Hungaro Duarte Department of Dentistry, Endodontics and Dental Materials, Bauru Dental School, University of São Paulo, Bauru, SP, Brazil
  • Clóvis Monteiro Bramante Department of Dentistry, Endodontics and Dental Materials, Bauru Dental School, University of São Paulo, Bauru, SP, Brazil
  • Flaviana Bombarda De Andrade Department of Dentistry, Endodontics and Dental Materials, Bauru Dental School, University of São Paulo, Bauru, SP, Brazil

DOI:

https://doi.org/10.1080/00016357.2016.1256427

Keywords:

Antibiotic, Enterococcus faecalis, calcium hydroxide, microscopy

Abstract

Objective: The aim of this study was to compare the in vitro intradentinal antimicrobial ability of the calcium hydroxide and tri-antibiotic pastes.

Materials and methods: Standard bovine dentin tubes were sterilized and then infected with Enterococcus faecalis by a new contamination protocol of great depths of dentin. The specimens were filled with the medications, divided into two test-groups: calcium hydroxide (Group 1) and tri-antibiotic (Group 2) pastes. After 15 days, the teeth were evaluated by microbiological culture and confocal laser scanning microscopy (CLSM) with viability dye assay LIVE/DEAD inside dentinal tubules. In experiment of culture, the bacterial collection of the dentin fragments was done for counting the colony-forming units.

Results: The tri-antibiotic paste had a slightly greater antimicrobial effect; however, there was no statistical difference between the groups.

Conclusions: It was concluded that the tri-antibiotic paste and the calcium hydroxide paste exercise the same effect on intra-tubular decontamination against E. faecalis. So, due the multiples advantages, the calcium hydroxide paste can be the choice for dentinal decontamination in regenerative procedures.

Downloads

Download data is not yet available.

Downloads

Published

2017-02-17

Issue

Vol. 75 No. 2 (2017): Acta Odontologica Scandinavica

Section

Articles