Enlargement of the apical foramen of mature teeth by instrumentation and apicoectomy. A study of effectiveness and the formation of dentinal cracks

Abstract

Objective: In the last few years there have been attempts to revascularize mature necrotic teeth instead of performing a standard root canal treatment. Apical foramen enlargement (AFE) would be necessary for regenerative treatments of mature teeth. In the literature, AFE has been made through apicoectomy and instrumentation. However, no standardized methods have been described yet, which may affect the success of the therapy. Our aim was to describe the effectiveness and damage to dental structures of five methods for AFE.

Methods: Two hundred and ten human teeth were assigned to one control group (n = 10) and four treatment groups (n = 50 each): instrumentation was up to file #80 0.5 mm coronal to the apex (I), at apex level (II), 0.5 mm beyond the apex (III) and apicoectomy at 2 and 4 mm from the apex (IV). The apical foramen diameter was measured before and after treatment. The formation of clinically visible fractures (CVF) and microcracks was analysed clinically and with ESEM, respectively. Thirty-two in situ sheep’s teeth were also instrumented, to compare damage in in situ and ex vivo teeth.

Results: The foramen diameter was augmented by 0.15, 0.47, 0.54 0.06 and 0.32 mm in human teeth of groups I, II, III, apicoectomy at 2 and 4 mm, respectively. CVF were more frequent as the working length was augmented. No statistical differences were found for microcrack formation. In situ teeth showed significantly less damage.

Conclusions: Instrumentation at apex level seems to be the most effective and least harmful technique for AFE, while apicoectomy is not a useful method.