Durability of the anti-erosive effect of surfaces sealants under erosive abrasive conditions

Abstract

Objective. To test the durability of sealants applied for prevention of erosive dentine mineral loss under erosive/abrasive conditions. Methods. Forty-eight bovine dentine samples doped with ³²P were randomly allocated to four groups (1–4). All samples performed a de- and remineralizations pre-cycling (6 × 1 min erosion in HCl: pH 3.0, mean time and overnight immersion in artificial saliva) for 1 day. Sealing was done as follows; (1) unsealed, (2) Seal & Protect, (3) K-0184 (experimental sealer) and (4) OptiBond FL. After sealing, samples were immersed in HCl for 3 h (baseline measurement). Then, the following erosive/abrasive and remineralisations cycling was performed for 8 days: 3 h/day erosion with HCl, 600 brushing strokes/day and storage in artificial saliva for the rest of the day. Sealer permeability was evaluated by assignation of ³²P in the acid used for the erosive attacks. Results. At baseline, the significantly highest dentine loss was observed for the unsealed control group, while the mineral loss was not statistically significantly different between the sealed groups 2 and 3. At all days of the erosive/abrasive and remineralizations cycling and cumulatively the significantly highest mineral loss was observed for group 1, while the significantly lowest mineral loss was observed for the samples sealed with Seal & Protect (group 2) and K-0184 (group 3). In all groups, no significant increase in the ³²P release was observed. Conclusion. Surface sealants are able to reduce the erosive dentine mineral loss and maintain this erosion-preventing efficacy over the whole duration (simulating 8 month in-vivo) of the erosive/abrasive cycling.