Quantitation of IgA in Human Whole Saliva

A Comparison of Three Immunoassays

Authors

  • S. Alaluusua Department of Pedodontics and Orthodontics, Institute of Dentistry, University of Helsinki, Finland
  • E. A. Grönblad Department of Bacteriology and Immunology, University of Helsinki, Finland
  • H. Tölö Scientific Laboratory, Institute of Dentistry, University of Helsinki, Finland

DOI:

https://doi.org/10.3109/00016358109162274

Keywords:

Solid phase radio-immunoassay, immunoelectrophoresis, single radial immunodiffusion

Abstract

Abstract

Concentration of IgA was measured in saliva samples from 23 healthy adults by solid phase radioimmunoassay (RIA), rocket Immunoelectrophoresis (IEF) and single gradial immunodiffusion (SRID). In preliminary experiments it was found that all three methods gave too low values of salivary IgA unless the samples were reduced with dithiothreitol.

After such reduction the mean concentrations of IgA in whole saliva were 100 mg/l (log mean 2.01 ± 0.22) measured with RIA, 90 mg/l (log mean 1.95 ± 0.18) measured with IEF and 120 mg/l (log mean 2.06 ± 0.19) measured with SRID.

Downloads

Download data is not yet available.

Downloads

Published

1981-01-01

Issue

Vol. 39 No. 3 (1981): Acta Odontologica Scandinavica

Section

Articles