Virulence genes of Porphyromonas gingivalis W83 in chronic periodontitis

Authors

  • Li Lin Department of Periodontics, School of Stomatology, China Medical University, Shenyang, China
  • Chen Li Department of Periodontics, School of Stomatology, China Medical University, Shenyang, China
  • Jingbo Liu Department of Periodontics, School of Stomatology, China Medical University, Shenyang, China
  • Dongmei Zhang Department of Periodontics, School of Stomatology, China Medical University, Shenyang, China
  • Jian Zhao Department of Periodontics, School of Stomatology, China Medical University, Shenyang, China
  • Yurong Kou Department of Periodontics, School of Stomatology, China Medical University, Shenyang, China
  • Ning Yu Department of Periodontics, School of Stomatology, China Medical University, Shenyang, China
  • Yaping Pan Department of Periodontics, School of Stomatology, China Medical University, Shenyang, China

Keywords:

DNA, microarray, suppression subtractive hybridization

Abstract

Objective. To identify virulence genes found in highly virulent strains of Porphyromonas gingivalis (P. gingivalis) among Chinese patients with chronic periodontitis and to evaluate the association of these virulence genes with clinical parameters and with periodontal tissue destruction. Material and methods.Suppression subtractive hybridization was applied to acquire short gene fragments harbored only in virulent strains of P. gingivalis W83. Eighteen genes, which were present in P. gingivalis W83 but absent from P. gingivalis ATCC 33277, were labeled with Cy5 and used as probes in DNA microarray hybridization to analyze DNA of P. gingivalis isolated from chronic periodontitis patients. Results. Spearman correlation analysis revealed 10 genes correlated with probing depth, clinical attachment loss, and tooth mobility (p<0.05). Conclusion. These genes may provide an important clue towards our understanding the mechanism of occurrence and the development of periodontal disease.

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Published

2009-01-01

Issue

Vol. 67 No. 5 (2009): Acta Odontologica Scandinavica

Section

Articles