Characterization of saliva proteins from ‘stainers’ and ‘non-stainers’ adsorbed to hydroxyapatite

Authors

  • Harald M. Eriksen Departments of Operative Dentistry and Pedodontics, Dental Faculty, and Department of Biochemistry, Faculty of Mathematics and Natural Sciences, University of Oslo, Oslo, Norway
  • Heidi Kantanen Departments of Operative Dentistry and Pedodontics, Dental Faculty, and Department of Biochemistry, Faculty of Mathematics and Natural Sciences, University of Oslo, Oslo, Norway
  • Håkon Nordbø Departments of Operative Dentistry and Pedodontics, Dental Faculty, and Department of Biochemistry, Faculty of Mathematics and Natural Sciences, University of Oslo, Oslo, Norway
  • Kirsten Hannesson Eggen Departments of Operative Dentistry and Pedodontics, Dental Faculty, and Department of Biochemistry, Faculty of Mathematics and Natural Sciences, University of Oslo, Oslo, Norway
  • Knut Sletten Departments of Operative Dentistry and Pedodontics, Dental Faculty, and Department of Biochemistry, Faculty of Mathematics and Natural Sciences, University of Oslo, Oslo, Norway

DOI:

https://doi.org/10.3109/00016358509046496

Keywords:

Amino acid analyses, dental pellicle, ion-exchange chromatography, tooth discoloration

Abstract

Stainers and non-stainers were selected on the basis of their individual tendency to develop extrinsic tooth discolorations from a chlorhexidine mouth rinse. Saliva proteins adsorbed to hydroxyapatite in vitro and in vivo pellicle from the participants were analyzed by gel filtration and ion-exchange chromatography. Two distinct anionic components were isolated. The elution patterns from stainers and non-stainers were identical. Amino acid analyses of the main peaks demonstrated a prevalence of serine, glycine, and glutamic acid.

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Published

1985-01-01

Issue

Vol. 43 No. 2 (1985): Acta Odontologica Scandinavica

Section

Articles