Electron immunoperoxidase staining in sections of unfixed pure cultures of Streptococcus mutans

Authors

  • Peter Berthold Department of Prosthetics, Karolinska In-stitutet, Stockholm, Sweden
  • C.-H. Berthold Department of Oral Microbiology, Karolinska In-stitutet, Stockholm, Sweden
  • C.-E. Nord Department of Anatomy School of Medicine, University of Gothenburg, Gothenburg, Sweden

DOI:

https://doi.org/10.3109/00016358209024071

Keywords:

Electron microscopy, fixation, immunohistochemistry

Abstract

Unfixed as well as glutaraldehyde-fixed pelleted cells from pure cultures of Streptococcus mutans subspecies sobrinus (B13) were embedded in glycolme-thacrylate. Uitrathin were sections immunoperoxidase labelled with rabbit anti-S. mutans sobrinus γ-globulin. It was found that unfixed specimens became labelled as precisely and as distinctly as did the fixed specimens. All controls were negative.

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Published

1982-01-01

Issue

Vol. 40 No. 5 (1982): Acta Odontologica Scandinavica

Section

Articles