A simple system for generating low-dosage mercury vapor for animal experiments

Abstract

A system using unlabeled, metallic mercury for generating low-dosage vapor for animal experiments is described. The system consists of four acrylic plastic chambers: one chamber containing the mercury source, one for mixing the mercury vapor with air, one exposure chamber, and one containing activated coal filters and mechanisms for regulating the airflow. The chambers are connected to each other by means of 80-mm-diameter polyvinyl chloride tubes reinforced with wire. Additional control and supporting equipment is also used. The system is easy to set up and requires minimal attendance during use. A standard deviation of 3–4% in the mercury vapor level during 6 h of exposure is typical. The conditions for the animals during exposure and the safety for the personnel during use are optimal.