Measurement of the horny layer turnover after staining with dansyl chloride: description of a new method

Authors

  • A Johannesson
  • H Hammar

DOI:

https://doi.org/10.2340/00015555587679

Abstract

A simple non-radioactive technique is described. The corneal layers were stained with 5% dansyl chloride ointment during 24-48 h of occlusion. The number of newly formed corneal layers were counted on sections from punch biopsies obtained at various intervals after the staining. The rate of formation is given as layers formed per 24 hours. The technique makes it possible to detect an increase in the turnover rate when it is more than 15-32% above normal. The biological material can also be utilized for comparative studies utilizing biochemical and histological methods. The average rate of formation was 1.15+-0.09 (S.E.M.) layers per 24 hours in a group of 10 subjects.

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Published

1978-01-01

How to Cite

Johannesson, A., & Hammar, H. (1978). Measurement of the horny layer turnover after staining with dansyl chloride: description of a new method. Acta Dermato-Venereologica, 58(1), 76–79. https://doi.org/10.2340/00015555587679

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Section

Articles