Increased Levels of Interleukin-17A Exosomes in Psoriasis

Authors

  • Claire Jacquin-Porretaz
  • Marine Cordonnier
  • Charlée Nardin
  • Laura Boullerot
  • Gaetan Chanteloup
  • Valentin Vautrot
  • Olivier Adotevi
  • Carmen Garrido
  • Jessica Gobbo
  • François Aubin

DOI:

https://doi.org/10.2340/00015555-3300

Keywords:

exosomes, psoriasis, IL-17

Abstract

Exosomes are involved in modulating the immune system and mediating communication between cells. The aim of this study was to investigate the involvement of exosomes in psoriasis. Exosomes from patients with psoriasis were analysed by nanoparticle tracking analysis and protein expression was analysed by western blotting. The concentration of HSP70 was determined by an enzyme-linked immunosorbent assay, and concentrations of interleukin (IL)-1?, IL-2, IL-6, IL-10, IL-17A and tumour necrosis factor alpha (TNF-?) were determined by flow cytometry. Based on the severity of psoriasis, evaluated by body surface area (??10% vs. >?10%), 2 groups of patients were compared (49 with mild psoriasis and 71 with moderate-to-severe psoriasis). The number (2.52?1011???2.29?1010 vs. 1.79?1011???1.93?1010, p?=?0.19) and size (94.44???22.00 nm vs. 96.87???28.30 nm, p?=?0.72) of exosomes and the concentration of HSP70 in the exosomes were not significantly different in the 2 groups of patients. IL-17A exosome levels were significantly higher in patients with moderate-to-severe psoriasis compared with those with mild psoriasis (p?=?0.02). There were no significant differences in levels of TNF-?, IL-1, IL-2, IL-6 and IL-10. This study shows, for the first time, the presence of circulating exosomes in patients with psoriasis. These data confirm the involvement of circulating exosomes in psoriasis, in particular in moderate-to-severe psoriasis, through IL-17A-producing exosomes.

Downloads

Download data is not yet available.

Downloads

Published

2019-09-23

How to Cite

Jacquin-Porretaz, C., Cordonnier, M., Nardin, C., Boullerot, L., Chanteloup, G., Vautrot, V., … Aubin, F. (2019). Increased Levels of Interleukin-17A Exosomes in Psoriasis. Acta Dermato-Venereologica, 99(12), 1143–1147. https://doi.org/10.2340/00015555-3300

Issue

Section

Articles