Evaluating the use of self-conditioning adhesive combined with dual curing resin cement as an endodontic sealer: An in vitro study.

Clara Isabel Anton y Otero; Nicolas Liaudet; Enrico di Bella; Marwa Abdelaziz; Albert Feilzer; Ivo Krejci; Laurine Marger

doi:10.1080/26415275.2023.2282523

Authors

Clara Isabel Anton y Otero Division of Cariology and Endodontology, CUMD - University Clinics of Dental Medicine, Faculty of Medicine, University of Geneva, Geneva, Switzerland
Nicolas Liaudet Bioimaging Core Facility, University of Geneva, Geneva, Switzerland
Enrico di Bella Division of Cariology and Endodontology, CUMD - University Clinics of Dental Medicine, Faculty of Medicine, University of Geneva, Geneva, Switzerland
Marwa Abdelaziz Division of Cariology and Endodontology, CUMD - University Clinics of Dental Medicine, Faculty of Medicine, University of Geneva, Geneva, Switzerland
Albert Feilzer Department of Dental Material Sciences, Academic Center for Dentistry Amsterdam (ACTA), Gustav Mahlerlaan, LA Amsterdam
Ivo Krejci Division of Cariology and Endodontology, CUMD - University Clinics of Dental Medicine, Faculty of Medicine, University of Geneva, Geneva, Switzerland
Laurine Marger Division of Cariology and Endodontology, CUMD - University Clinics of Dental Medicine, Faculty of Medicine, University of Geneva, Geneva, Switzerland

DOI:

https://doi.org/10.1080/26415275.2023.2282523

Keywords:

composite resin, sealer, adhesion, cement, infiltration, microleakage, confocal microscopy, endodontics, dentin

Abstract

Objectives

To compare infiltration, sealing and microleakage in root dentin with a self-conditioning adhesive system combined with dual curing resin (resin-based cement) to a conventional epoxy-resin-based sealer using confocal microscopy imaging.

Methods

26 roots were enlarged and disinfected. Dentin tubules of 24 teeth were labelled with a red fluorophore (Rhodamine B) (two samples served as controls). Root canal samples were sealed in group AH (n = 11) with a conventional sealer (AH Plus Root Canal Sealer, Dentsply DeTrey) and in group RC (n = 11) with a resin-based cement (Parabond combined with Paracore, Coltène). Roots were then sectioned horizontally and immersed in H₂O₂ to remove the Rhodamine B not fixed by the sealers. The empty dentin spaces were labeled with a green fluorophore (Fluorescein) enabling the evaluation of infiltration as well as microleakage by confocal microscopy. Two additional samples were fractured in vertical direction for observation under SEM.

Results

Group RC presented significantly more infiltration in the middle third than in the middle and apical thirds of group AH. Microleakage was significantly higher in group AH than in group RC. SEM images revealed more dentin plugs and a homogenous resin layer in group RC in contrast to group AH.

Conclusion

The resin-based cement revealed promising outcomes compared to a traditional epoxy resin based sealer.

Key messages

Infiltration and microleakage in infiltrated and sealed root dentin samples are higher in middle than apical root thirds.

Root dentin infiltration and sealing with a self-conditioning adhesive system and a dual-curing resin cement revealed less microleakage than with an epoxy-resin-based sealer.