The in vitro assessment of resin coating materials containing calcium phosphate, bioactive glass, and polylysine for glass ionomer cement restorations
DOI:
https://doi.org/10.2340/biid.v12.42783Keywords:
Glass ionomer cements, degree of monomer conversion, resin coating, fluoride release, biaxial flexural strengthAbstract
Objective: Glass ionomer cements (GICs) require protective surface coatings to enhance their clinical performance. This study developed novel protective resin coatings for GICs containing monocalcium phosphate monohydrate (MCPM), bioactive glass nanoparticles (BAGs), and poly-L-lysine (PLS) and evaluated their physical, mechanical, and biological properties when applied to GICs.
Materials and methods: Experimental resin coating materials were formulated with 5–10 wt% of MCPM, BAGs, and PLS. The degree of monomer conversion was measured usingAttenuated total reflectance-Fourier transform infrared spectroscopy (ATR-FTIR) (n = 6). GICs coated with the experimental materials were evaluated for biaxial flexural strength and modulus after 24 h water immersion using a universal testing machine (n = 8). Vickers surface microhardness up to 4 weeks of water immersion was also determined (n = 5). Fluoride and elemental release in water were analyzed using a fluoride-specific electrode and inductively coupled plasma optical emission spectrometry (n = 3). Cell viability was assessed using an MTT assay with mouse fibrosarcoma (n = 3). A commercial resin coating (EQUIA Forte Coat, EQ) served as control. Data were analyzed using one-way ANOVA and Tukey HSD test.
Results: While EQ showed higher monomer conversion (87%) compared to experimental materials (72–74%) (p < 0.05), GICs coated with experimental materials demonstrated comparable strength to EQ-coated GICs. The experimental coatings exhibited similar F, Al, Na, and Si releases to EQ-coated GICs, with enhanced P release. All experimental coatings exhibited comparable cell viability (>70%) to the commercial material.
Conclusion: The novel GIC protective coatings containing MCPM, BAGs, and PLS demonstrated acceptable in vitro performance comparable to commercial materials while potentially offering enhanced remineralization through increased elemental release.
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Cribari L, Madeira L, Roeder RBR, Macedo RM, Wambier LM, Porto TS, et al. High-viscosity glass-ionomer cement or composite resin for restorations in posterior permanent teeth? A systematic review and meta-analyses. J Dent. 2023;137:104629. https://doi.org/10.1016/j.jdent.2023.104629 DOI: https://doi.org/10.1016/j.jdent.2023.104629
Ge KX, Quock R, Chu CH, Yu OY. The preventive effect of glass ionomer cement restorations on secondary caries formation: a systematic review and meta-analysis. Dent Mater. 2023;39(12):e1–17. https://doi.org/10.1016/j.dental.2023.10.008 DOI: https://doi.org/10.1016/j.dental.2023.10.008
Vetromilla BM, Opdam NJ, Leida FL, Sarkis-Onofre R, Demarco FF, van der Loo MPJ, et al. Treatment options for large posterior restorations: a systematic review and network meta-analysis. J Am Dent Assoc. 2020;151(8):614–24.e618. https://doi.org/10.1016/j.adaj.2020.05.006 DOI: https://doi.org/10.1016/j.adaj.2020.05.006
Gurgan S, Kutuk ZB, Yalcin Cakir F, Ergin E. A randomized controlled 10 years follow up of a glass ionomer restorative material in class I and class II cavities. J Dent. 2020;94:103175. https://doi.org/10.1016/j.jdent.2019.07.013 DOI: https://doi.org/10.1016/j.jdent.2019.07.013
Panpisut P, Toneluck A. Monomer conversion, dimensional stability, biaxial flexural strength, and fluoride release of resin-based restorative material containing alkaline fillers. Dent Mater J. 2020;39(4):608–15. https://doi.org/10.4012/dmj.2019-020 DOI: https://doi.org/10.4012/dmj.2019-020
van Dijken JWV, Pallesen U, Benetti A. A randomized controlled evaluation of posterior resin restorations of an altered resin modified glass-ionomer cement with claimed bioactivity. Dent Mater. 2019;35(2):335–43. https://doi.org/10.1016/j.dental.2018.11.027 DOI: https://doi.org/10.1016/j.dental.2018.11.027
Kielbassa AM, Oehme EP, Shakavets N, Wolgin M. In vitro wear of (resin-coated) high-viscosity glass ionomer cements and glass hybrid restorative systems. J Dent. 2021;105:103554. https://doi.org/10.1016/j.jdent.2020.103554 DOI: https://doi.org/10.1016/j.jdent.2020.103554
Heck K, Frasheri I, Diegritz C, Manhart J, Hickel R, Fotiadou C. Six-year results of a randomized controlled clinical trial of two glass ionomer cements in class II cavities. J Dent. 2020;97:103333. https://doi.org/10.1016/j.jdent.2020.103333 DOI: https://doi.org/10.1016/j.jdent.2020.103333
Tyagi S, Thomas AM, Sinnappah-Kang ND. A comparative evaluation of resin- and varnish-based surface protective agents on glass ionomer cement – a spectrophotometric analysis. Biomater Investig Dent. 2020;7(1):25–30. https://doi.org/10.1080/26415275.2020.1711760 DOI: https://doi.org/10.1080/26415275.2020.1711760
Diem VT, Tyas MJ, Ngo HC, Phuong LH, Khanh ND. The effect of a nano-filled resin coating on the 3-year clinical performance of a conventional high-viscosity glass-ionomer cement. Clin Oral Investig. 2014;18(3):753–9. https://doi.org/10.1007/s00784-013-1026-z DOI: https://doi.org/10.1007/s00784-013-1026-z
Panpisut P, Toneluck A, Khamsuk C, Channasanon S, Tanodekaew S, Monmaturapoj N, et al. The development of resin-coating materials for enhancing elemental release of coated glass ionomer cements. Heliyon. 2024;10(14):e34512. https://doi.org/10.1016/j.heliyon.2024.e34512 DOI: https://doi.org/10.1016/j.heliyon.2024.e34512
Thongbai-On N, Banomyong D. Flexural strengths and porosities of coated or uncoated, high powder-liquid and resin-modified glass ionomer cements. J Dent Sci. 2020;15(4):433–6. https://doi.org/10.1016/j.jds.2020.02.004 DOI: https://doi.org/10.1016/j.jds.2020.02.004
Krajangta N, Dulsamphan C, Chotitanmapong T. Effects of protective surface coating on fluoride release and recharge of recent uncoated high-viscosity glass ionomer cement. Dent J (Basel). 2022;10(12):233. https://doi.org/10.3390/dj10120233 DOI: https://doi.org/10.3390/dj10120233
Brzovic-Rajic V, Miletic I, Gurgan S, Peros K, Verzak Z, Ivanisevic-Malcic A. Fluoride release from glass ionomer with nano filled coat and varnish. Acta Stomatol Croat. 2018;52(4):307–13. https://doi.org/10.15644/asc52/4/4 DOI: https://doi.org/10.15644/asc52/4/4
Par M, Gubler A, Attin T, Tarle Z, Tarle A, Prskalo K, et al. Effect of adhesive coating on calcium, phosphate, and fluoride release from experimental and commercial remineralizing dental restorative materials. Sci Rep. 2022;12(1):10272. https://doi.org/10.1038/s41598-022-14544-9 DOI: https://doi.org/10.1038/s41598-022-14544-9
Imazato S, Nakatsuka T, Kitagawa H, Sasaki JI, Yamaguchi S, Ito S, et al. Multiple-ion releasing bioactive surface pre-reacted glass-ionomer (S-PRG) filler: innovative technology for dental treatment and care. J Funct Biomater. 2023;14(4):236. https://doi.org/10.3390/jfb14040236 DOI: https://doi.org/10.3390/jfb14040236
Panpisut P, Liaqat S, Zacharaki E, Xia W, Petridis H, Young AM. Dental composites with calcium/strontium phosphates and polylysine. PLoS One. 2016;11(10):e0164653. https://doi.org/10.1371/journal.pone.0164653 DOI: https://doi.org/10.1371/journal.pone.0164653
Alkhouri N, Xia W, Ashley P, Young A. The effect of varying monocalcium phosphate and polylysine levels on dental composite properties. J Mech Behav Biomed Mater. 2023;145:106039. https://doi.org/10.1016/j.jmbbm.2023.106039 DOI: https://doi.org/10.1016/j.jmbbm.2023.106039
Chaichana W, Insee K, Chanachai S, Benjakul S, Aupaphong V, Naruphontjirakul P, et al. Physical/mechanical and antibacterial properties of orthodontic adhesives containing Sr-bioactive glass nanoparticles, calcium phosphate, and andrographolide. Sci Rep. 2022;12(1):6635. https://doi.org/10.1038/s41598-022-10654-6 DOI: https://doi.org/10.1038/s41598-022-10654-6
Dai LL, Mei ML, Chu CH, Lo ECM. Antibacterial effect of a new bioactive glass on cariogenic bacteria. Arch Oral Biol. 2020;117:104833. https://doi.org/10.1016/j.archoralbio.2020.104833 DOI: https://doi.org/10.1016/j.archoralbio.2020.104833
Galarraga-Vinueza ME, Mesquita-Guimaraes J, Magini RS, Souza JC, Fredel MC, Boccaccini AR. Anti-biofilm properties of bioactive glasses embedding organic active compounds. J Biomed Mater Res A. 2017;105(2):672–9. https://doi.org/10.1002/jbm.a.35934 DOI: https://doi.org/10.1002/jbm.a.35934
Nedeljkovic I, Yoshihara K, De Munck J, Teughels W, Van Meerbeek B, Van Landuyt KL. No evidence for the growth-stimulating effect of monomers on cariogenic Streptococci. Clin Oral Investig. 2017;21(5):1861–9. https://doi.org/10.1007/s00784-016-1972-3 DOI: https://doi.org/10.1007/s00784-016-1972-3
Gosavi SS, Gosavi SY, Alla RK. Local and systemic effects of unpolymerised monomers. Dent Res J (Isfahan). 2010;7(2):82–7.
Lygidakis NN, Allan E, Xia W, Ashley PF, Young AM. Early polylysine release from dental composites and its effects on planktonic Streptococcus mutans growth. J Funct Biomater. 2020;11(3):53. https://doi.org/10.3390/jfb11030053 DOI: https://doi.org/10.3390/jfb11030053
Li S, Mao Y, Zhang L, Wang M, Meng J, Liu X, et al. Recent advances in microbial epsilon-poly-L-lysine fermentation and its diverse applications. Biotechnol Biofuels Bioprod. 2022;15(1):65. https://doi.org/10.1186/s13068-022-02166-2 DOI: https://doi.org/10.1186/s13068-022-02166-2
Potiprapanpong W, Naruphontjirakul P, Khamsuk C, Channasanon S, Toneluck A, Tanodekaew S, et al. Assessment of mechanical/chemical properties and cytotoxicity of resin-modified glass ionomer cements containing Sr/F-bioactive glass nanoparticles and methacrylate functionalized polyacids. Int J Mol Sci. 2023;24(12):10231. https://doi.org/10.3390/ijms241210231 DOI: https://doi.org/10.3390/ijms241210231
Panpisut P, Praesuwatsilp N, Bawornworatham P, Naruphontjirakul P, Patntirapong S, Young AM. Assessment of physical/mechanical performance of dental resin sealants containing Sr-bioactive glass nanoparticles and calcium phosphate. Polymers (Basel). 2022;14(24):5436. https://doi.org/10.3390/polym14245436 DOI: https://doi.org/10.3390/polym14245436
Delgado AHS, Young AM. Methacrylate peak determination and selection recommendations using ATR-FTIR to investigate polymerisation of dental methacrylate mixtures. PLoS One. 2021;16(6):e0252999. https://doi.org/10.1371/journal.pone.0252999 DOI: https://doi.org/10.1371/journal.pone.0252999
Walters NJ, Xia W, Salih V, Ashley PF, Young AM. Poly(propylene glycol) and urethane dimethacrylates improve conversion of dental composites and reveal complexity of cytocompatibility testing. Dent Mater. 2016;32(2):264–77. https://doi.org/10.1016/j.dental.2015.11.017 DOI: https://doi.org/10.1016/j.dental.2015.11.017
Thepveera W, Potiprapanpong W, Toneluck A, Channasanon S, Khamsuk C, Monmaturapoj N, et al. Rheological properties, surface microhardness, and dentin shear bond strength of resin-modified glass ionomer cements containing methacrylate-functionalized polyacids and spherical pre-reacted glass fillers. J Funct Biomater. 2021;12(3):42. https://doi.org/10.3390/jfb12030042 DOI: https://doi.org/10.3390/jfb12030042
Thanyasiri S, Naruphontjirakul P, Padunglappisit C, Mirchandani B, Young AM, Panpisut P. Assessment of physical/mechanical properties and cytotoxicity of dual-cured resin cements containing Sr-bioactive glass nanoparticles and calcium phosphate. Dent Mater J. 2023;42(6):806–17. https://doi.org/10.4012/dmj.2023-127 DOI: https://doi.org/10.4012/dmj.2023-127
British Standard. 10993–5: 2009 Biological evaluation of medical devices. In: Part 5: tests for in vitro cytotoxicity. London: BSI Standards Limited; 2009.
Pagano S, Lombardo G, Balloni S, Bodo M, Cianetti S, Barbati A, et al. Cytotoxicity of universal dental adhesive systems: assessment in vitro assays on human gingival fibroblasts. Toxicol In Vitro. 2019;60:252–60. https://doi.org/10.1016/j.tiv.2019.06.009 DOI: https://doi.org/10.1016/j.tiv.2019.06.009
Panpisut P, Khan MA, Main K, Arshad M, Xia W, Petridis H, et al. Polymerization kinetics stability, volumetric changes, apatite precipitation, strontium release and fatigue of novel bone composites for vertebroplasty. PLoS One. 2019;14(3):e0207965. https://doi.org/10.1371/journal.pone.0207965 DOI: https://doi.org/10.1371/journal.pone.0207965
Sideridou I, Tserki V, Papanastasiou G. Effect of chemical structure on degree of conversion in light-cured dimethacrylate-based dental resins. Biomaterials. 2002;23(8):1819–29. https://doi.org/10.1016/S0142-9612(01)00308-8 DOI: https://doi.org/10.1016/S0142-9612(01)00308-8
Elfakhri F, Alkahtani R, Li C, Khaliq J. Influence of filler characteristics on the performance of dental composites: a comprehensive review. Ceram Int. 2022;48(19):27280–94. https://doi.org/10.1016/j.ceramint.2022.06.314 DOI: https://doi.org/10.1016/j.ceramint.2022.06.314
Moldovan M, Balazsi R, Soanca A, Roman A, Sarosi C, Prodan D, et al. Evaluation of the degree of conversion, residual monomers and mechanical properties of some light-cured dental resin composites. Materials (Basel). 2019;12(13):2109. https://doi.org/10.3390/ma12132109 DOI: https://doi.org/10.3390/ma12132109
Lugović-Mihić L, Filija E, Varga V, Premuž L, Parać E, Tomašević R, et al. Unwanted skin reactions to acrylates: an update. Cosmetics. 2024;11(4):127. https://doi.org/10.3390/cosmetics11040127 DOI: https://doi.org/10.3390/cosmetics11040127
Pemberton MA, Lohmann BS. Risk assessment of residual monomer migrating from acrylic polymers and causing allergic contact dermatitis during normal handling and use. Regul Toxicol Pharmacol. 2014;69(3):467–75. https://doi.org/10.1016/j.yrtph.2014.05.013 DOI: https://doi.org/10.1016/j.yrtph.2014.05.013
Cadenaro M, Antoniolli F, Codan B, Agee K, Tay FR, Dorigo Ede S, et al. Influence of different initiators on the degree of conversion of experimental adhesive blends in relation to their hydrophilicity and solvent content. Dent Mater. 2010;26(4):288–94. https://doi.org/10.1016/j.dental.2009.11.078 DOI: https://doi.org/10.1016/j.dental.2009.11.078
Borges AFS, Chase MA, Guggiari AL, Gonzalez MJ, Ribeiro ARdS, Pascon FM, et al. A critical review on the conversion degree of resin monomers by direct analyses. Braz Dent Sci. 2013;16(1):18–26. https://doi.org/10.14295/bds.2013.v16i1.845 DOI: https://doi.org/10.14295/bds.2013.v16i1.845
Fonseca AS, Labruna Moreira AD, de Albuquerque PP, de Menezes LR, Pfeifer CS, Schneider LF. Effect of monomer type on the CC degree of conversion, water sorption and solubility, and color stability of model dental composites. Dent Mater. 2017;33(4):394–401. https://doi.org/10.1016/j.dental.2017.01.010 DOI: https://doi.org/10.1016/j.dental.2017.01.010
Kraigsley AM, Tang K, Lippa KA, Howarter JA, Lin-Gibson S, Lin NJ. Effect of polymer degree of conversion on Streptococcus mutans biofilms. Macromol Biosci. 2012;12(12):1706–13. https://doi.org/10.1002/mabi.201200214 DOI: https://doi.org/10.1002/mabi.201200214
Fu SY, Feng XQ, Lauke B, Mai YW. Effects of particle size, particle/matrix interface adhesion and particle loading on mechanical properties of particulate-polymer composites. Compos B Eng. 2008;39(6):933–61. https://doi.org/10.1016/j.compositesb.2008.01.002 DOI: https://doi.org/10.1016/j.compositesb.2008.01.002
Moghimi M, Jafarpour D, Ferooz R, Bagheri R. Protective effect of a nanofilled resin-based coating on wear resistance of glass ionomer cement restorative materials. BMC Oral Health. 2022;22(1):317. https://doi.org/10.1186/s12903-022-02347-3 DOI: https://doi.org/10.1186/s12903-022-02347-3
Nguyen LG, Kopperud HM, Øilo M. Water sorption and solubility of polyamide denture base materials. Acta Biomater Odontol Scand. 2017;3(1):47–52. https://doi.org/10.1080/23337931.2017.1326009 DOI: https://doi.org/10.1080/23337931.2017.1326009
Ferracane JL. Hygroscopic and hydrolytic effects in dental polymer networks. Dent Mater. 2006;22(3):211–22. https://doi.org/10.1016/j.dental.2005.05.005 DOI: https://doi.org/10.1016/j.dental.2005.05.005
Aljabo A, Xia W, Liaqat S, Khan MA, Knowles JC, Ashley P, et al. Conversion, shrinkage, water sorption, flexural strength and modulus of re-mineralizing dental composites. Dent Mater. 2015;31(11):1279–89. https://doi.org/10.1016/j.dental.2015.08.149 DOI: https://doi.org/10.1016/j.dental.2015.08.149
Luo J, Billington RW, Pearson GJ. Kinetics of fluoride release from glass components of glass ionomers. J Dent. 2009;37(7):495–501. https://doi.org/10.1016/j.jdent.2009.02.007 DOI: https://doi.org/10.1016/j.jdent.2009.02.007
Abdalla MM, Bijle MN, Abdallah NMA, Yiu CKY. Enamel remineralization potential and antimicrobial effect of a fluoride varnish containing calcium strontium silicate. J Dent. 2023;138:104731. https://doi.org/10.1016/j.jdent.2023.104731 DOI: https://doi.org/10.1016/j.jdent.2023.104731
Tripathi R, Yadav JP, Pathak P, Almatarneh MH, Verma A. Chapter 6 – polymer–drug linking through amide bonds: the chemistry and applications in drug delivery. In: Madan J, Baldi A, Chaudhary M, Chopra N, editors. Polymer-drug conjugates. Academic Press; London, United Kingdom: 2023. p. 147–70. DOI: https://doi.org/10.1016/B978-0-323-91663-9.00007-2
Alkhouri N, Xia W, Ashley PF, Young AM. Renewal MI dental composite etch and seal properties. Materials (Basel). 2022;15(15):5438. https://doi.org/10.3390/ma15155438 DOI: https://doi.org/10.3390/ma15155438
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