Comparing the stability of implants coated with nano PLGA-alendronate sodium: an in-vivo study
DOI:
https://doi.org/10.2340/biid.v12.43372Keywords:
PLGA-alendronate, implant anchorage, dip coatingAbstract
Objective: Considering the effect of bone resorption-inhibiting drugs, such as bisphosphonates, on bone density, the present study evaluated the stability of implants coated with polylactic-co-glycolic acid (PLGA)-alendronate sodium.
Methodology: Ten healthy Swiss rabbits were selected (mean weight: 3.5 kg). Two titanium implants were inserted in each rabbit’s tibia bone, one implant coated with PLGA-alendronate sodium and the other functioning as control. Torque meter equipment measured the amount of torque while inserting the implants. Insertion torque was measured during the initial insertion of the implants, and after 4 weeks, the rabbits were anesthetized again, the implants were exposed, and resistance torques were measured and recorded. Using a trephine bur, all implants and the surrounding bone structure were then removed for histological evaluation. Fourier transform infrared spectroscopy was used to investigate alendronate and PLGA biologically. Data analysis was performed using SPSS.v23 software with a significance level of p < 0.05.
Results: The results showed that the difference between insertion torque and final resistance torque after 4 weeks was statistically significant (p = 0.024). The mean number of osteoclasts and macrophages differed substantially between the experimental and control groups (p < 0.001). The ratio of woven bone to lamellar bone and the number of osteoblasts did not differ significantly between the two groups (p > 0.05).
Conclusion: The use of PLGA-alendronate sodium coating decreased the number of osteoclasts and macrophages and increased the stability of implant anchorage.
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Tuikampee S, Chaijareenont P, Rungsiyakull P, Yavirach A. Titanium surface modification techniques to enhance osteoblasts and bone formation for dental implants: a marrative review on current advances. Metals. 2024;14(5):515. https://doi.org/10.3390/met14050515
Oliveira ÉR, Nie L, Podstawczyk D, Allahbakhsh A, Ratnayake J, Brasil DL, et al. Advances in growth factor delivery for bone tissue engineering. Int J Mol Sci. 2021;22(2):903. https://doi.org/10.3390/ijms22020903
Esposito M, Worthington HV, Coulthard P. Interventions for replacing missing teeth: dental implants in zygomatic bone for the rehabilitation of the severely deficient edentulous maxilla. Cochrane Database Syst Rev. 2005;(4):CD004151. https://doi.org/10.1002/14651858.CD004151.pub2
Esposito M, Grusovin MG, Felice P, Karatzopoulos G, Worthington HV, Coulthard P. Interventions for replacing missing teeth: horizontal and vertical bone augmentation techniques for dental implant treatment. Cochrane Database Syst Rev. 2009;(4):CD003607. https://doi.org/10.1002/14651858.CD003607.pub4
Perinelli DR, Cespi M, Bonacucina G, Palmieri GF. PEGylated polylactide (PLA) and poly (lactic-co-glycolic acid)(PLGA) copolymers for the design of drug delivery systems. J Pharmaceut Investig. 2019;49:443–58. https://doi.org/10.1007/s40005-019-00442-2
Chavan Y, Tambe S, Jain D, Khairnar S, Amin P, editors. Redefining the importance of polylactide-co-glycolide acid (PLGA) in drug delivery. Ann Pharm Fr. 2022;80(5):603–16. https://doi.org/10.1016/j.pharma.2021.11.009
Greenwald RB, Yang K, Zhao H, Conover CD, Lee S, Filpula D. Controlled release of proteins from their poly (ethylene glycol) conjugates: drug delivery systems employing 1, 6-elimination. Bioconjugate Chem. 2003;14(2):395–403. https://doi.org/10.1021/bc025652m
Schmidmaier G, Wildemann B, Bail H, Lucke M, Fuchs T, Stemberger A, et al. Local application of growth factors (insulin-like growth factor-1 and transforming growth factor-β1) from a biodegradable poly (D, L-lactide) coating of osteosynthetic implants accelerates fracture healing in rats. Bone. 2001;28(4):341–50. https://doi.org/10.1016/S8756-3282(00)00456-7
Priyadarshini V, Maity S, Amruthesh A. Bisphosphonates and periodontics. J Cardiovasc Dis Res 2021;12:1899–908.
McCall RL, Sirianni RW. PLGA nanoparticles formed by single-or double-emulsion with vitamin E-TPGS. J Vis Exp. 2013;(82):51015. https://doi.org/10.3791/51015
Birang R, Shahabooei M, Mashhadiabbas F, Atabaki M, Naghsh N, Kavosh K, et al. Effect of enamel matrix derivative on bone formation around intraosseous titanium implant: an experimental study in canine model. Dent Res J. 2012;9(6):790–6. https://doi.org/10.4103/1735-3327.92961
Kajiwara H, Yamaza T, Yoshinari M, Goto T, Iyama S, Atsuta I, et al. The bisphosphonate pamidronate on the surface of titanium stimulates bone formation around tibial implants in rats. Biomaterials. 2005;26(6):581–7. https://doi.org/10.1016/j.biomaterials.2004.02.072
Despotović I, Petrović Ž, Katić J, Mikić D. Alendronate as bioactive coating on titanium surfaces: an investigation of CaP–alendronate interactions. Materials. 2024;17(11):2703. https://doi.org/10.3390/ma17112703
Meraw SJ, Reeve CM. Qualitative analysis of peripheral peri‐implant bone and influence of alendronate sodium on early bone regeneration. J Periodontol. 1999;70(10):1228–33. https://doi.org/10.1902/jop.1999.70.10.1228
Yaffe A, Iztkovich M, Earon Y, Alt I, Lilov R, Binderman I. Local delivery of an amino bisphosphonate prevents the resorptive phase of alveolar bone following mucoperiosteal flap surgery in rats. J Periodontol. 1997;68(9):884–9. https://doi.org/10.1902/jop.1997.68.9.884
Jin S, Xia X, Huang J, Yuan C, Zuo Y, Li Y, et al. Recent advances in PLGA-based biomaterials for bone tissue regeneration. Acta Biomater. 2021;127:56–79. https://doi.org/10.1016/j.actbio.2021.03.067
Aerssens J, Boonen S, Lowet G, Dequeker J. Interspecies differences in bone composition, density, and quality: potential implications for in vivo bone research. Endocrinology. 1998;139(2):663–70. https://doi.org/10.1210/endo.139.2.5751
Mir M, Ahmed N, ur Rehman A. Recent applications of PLGA based nanostructures in drug delivery. Coll Surf B Biointerf. 2017;159:217–31. https://doi.org/10.1016/j.colsurfb.2017.07.038
Martins C, Sousa F, Araujo F, Sarmento B. Functionalizing PLGA and PLGA derivatives for drug delivery and tissue regeneration applications. Adv Healthc Mater. 2018;7(1):1701035. https://doi.org/10.1002/adhm.201701035
Klara J, Lewandowska-Łańcucka J. How efficient are alendronate-nano/biomaterial combinations for anti-osteoporosis therapy? An evidence-based review of the literature. Int J Nanomed. 2022;17:6065. https://doi.org/10.2147/IJN.S388430
Park H. Exploring the effects of process parameters during W/O/W emulsion preparation and supercritical fluid extraction on the protein encapsulation and release properties of PLGA microspheres. Pharmaceutics. 2024;16(3):302. https://doi.org/10.3390/pharmaceutics16030302
Meraw SJ, Reeve CM, Wollan PC. Use of alendronate in peri‐implant defect regeneration. J Periodontol. 1999;70(2):151–8. https://doi.org/10.1902/jop.1999.70.2.151
Im G-I, Qureshi SA, Kenney J, Rubash HE, Shanbhag AS. Osteoblast proliferation and maturation by bisphosphonates. Biomaterials. 2004;25(18):4105–15. https://doi.org/10.1016/j.biomaterials.2003.V11.024
García-Recio E, Costela-Ruiz VJ, Illescas-Montes R, Melguizo-Rodríguez L, García-Martínez O, Ruiz C, et al. Modulation of osteogenic gene expression by human osteoblasts cultured in the presence of bisphenols BPF, BPS, or BPAF. Int J Mol Sci. 2023;24(5):4256. https://doi.org/10.3390/ijms24054256
Maruotti N, Corrado A, Neve A, Cantatore FP. Bisphosphonates: effects on osteoblast. Eur J Clin Pharmacol. 2012;68:1013–18. https://doi.org/10.1007/s00228-012-1216-7
Scala R, Maqoud F, Antonacci M, Dibenedetto JR, Perrone MG, Scilimati A, et al. Bisphosphonates targeting ion channels and musculoskeletal effects. Front Pharmacol. 2022;13:837534. https://doi.org/10.3389/fphar.2022.837534
Dr. Rogers MJ, Chilton KM, Coxon FP, Lawry J, Smith MO, Suri S, et al. Bisphosphonates induce apoptosis in mouse macrophage‐like cells in vitro by a nitric oxide‐independent mechanism. J Bone Miner Res. 1996;11(10):1482–91. https://doi.org/10.1002/jbmr.5650111015
Vertesich K, Sosa BR, Niu Y, Ji G, Suhardi V, Turajane K, et al. Alendronate enhances osseointegration in a murine implant model. J Orthopaed Res. 2021;39(4):719–26. https://doi.org/10.1002/jor.24853
Jolic M, Sharma S, Palmquist A, Shah FA. The impact of medication on osseointegration and implant anchorage in bone determined using removal torque – a review. Heliyon. 2022;8(10):e10844. https://doi.org/10.1016/j.heliyon.2022.e10844
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