Effects of Sr/F-bioactive glass nanoparticles on pH, elemental release, dentin remineralisation, and cytotoxicity of 1.1% NaF toothpaste
DOI:
https://doi.org/10.2340/biid.v12.44239Keywords:
Bioactive glass, 5000 ppm toothpaste, fluoride release, elemental release, cytotoxicity, remineralisationAbstract
Objective: This study examined the effect of Sr/F-bioactive glass nanoparticles (Sr/F-BAG) concentration on 1.1% NaF toothpaste. The effects of additives on pH, fluoride and elemental release, dentin remineralisation, and cytotoxicity were determined.
Materials and methods: Sr/F-BAG particles were incorporated into 1.1% NaF toothpaste (0, 1, 2, and 4 wt%). F release and pH upon immersion in deionised water were determined using a fluoride-specific electrode and pH meter (n = 8). Elemental release was analysed using Inductively Coupled Plasma Optical Emission Spectroscopy (n = 3). Dentin remineralisation (mineral-to-collagen ratio) after application of experimental toothpaste was compared using Attenuated Total Reflectance Fourier Transform Infrared Spectroscopy (ATR-FTIR, n = 9). Cytotoxicity was assessed using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay (n = 3). Colgate PreviDent 5000 Plus toothpaste (PV) was used as a commercial comparison.
Results: The addition of 0 to 4 wt% Sr/F-BAG linearly increased pH and F release of the 1.1% NaF toothpaste. Each 1 wt% increase in Sr/F-BAG concentration, raised pH by 0.3 and fluoride release by 457 ppm. The additives also enhanced the release of Ca, P, and Sr from the experimental toothpaste. At high concentration of Sr/F-BAG (4 wt%), the pH of the experimental toothpaste was comparable to PV (p > 0.05) but with significantly higher fluoride release (p < 0.05). However, PV demonstrated a significantly higher increase in mineral-to-collagen ratio compared to the experimental materials. The dentin surface treated with PV also showed more evident mineral precipitation. Furthermore, the experimental toothpaste containing 4 wt% Sr/F-BAG demonstrated higher cell viability (90%) than PV (56%).
Conclusion: The addition of Sr/F-BAG enhanced the release of F, Ca, P, Sr, and increased the pH of the toothpaste. However, the experimental toothpaste with added bioactive glass up to 4 wt% did not demonstrate superior remineralising effects compared to commercial 1.1% NaF toothpaste. In addition, the incorporation of Sr/F-BAG promoted the cytocompatibility of the experimental toothpaste.
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Marcenes W, Kassebaum NJ, Bernabe E, Flaxman A, Naghavi M, Lopez A, et al. Global burden of oral conditions in 1990–2010: a systematic analysis. J Dent Res. 2013;92(7):592–7. https://doi.org/10.1177/0022034513490168 DOI: https://doi.org/10.1177/0022034513490168
GBD Oral Disorders Collaborators. Global, regional, and national levels and trends in burden of oral conditions from 1990 to 2017: a systematic analysis for the global burden of disease 2017 study. J Dent Res. 2020;99(4):362–73. DOI: https://doi.org/10.1177/0022034520908533
Slayton RL, Urquhart O, Araujo MWB, Fontana M, Guzman-Armstrong S, Nascimento MM, et al. Evidence-based clinical practice guideline on nonrestorative treatments for carious lesions: a report from the American Dental Association. J Am Dent Assoc. 2018;149(10):837–49 e819. https://doi.org/10.1016/j.adaj.2018.07.002 DOI: https://doi.org/10.1016/j.adaj.2018.07.002
World Health Organization. Bangkok declaration – No health without oral health: towards universal health coverage for oral health by 2030. Bangkok, Thailand: 2025.
Benzian H. Dental public health breakthrough. Br Dent J 2022;232(7):421. https://doi.org/10.1038/s41415-022-4150-9 DOI: https://doi.org/10.1038/s41415-022-4150-9
Gkekas A, Varenne B, Stauf N, Benzian H, Listl S. Affordability of essential medicines: the case of fluoride toothpaste in 78 countries. PLoS One. 2022;17(10):e0275111. https://doi.org/10.1371/journal.pone.0275111 DOI: https://doi.org/10.1371/journal.pone.0275111
Walsh T, Worthington HV, Glenny AM, Marinho VC, Jeroncic A. Fluoride toothpastes of different concentrations for preventing dental caries. Cochrane Database Syst Rev 2019;3:CD007868. https://doi.org/10.1002/14651858.CD007868.pub3 DOI: https://doi.org/10.1002/14651858.CD007868.pub3
Prasad PS, Pasha MB, Rao RN, Rao PV, Madaboosi N, Ozcan M. A review on enhancing the life of teeth by toothpaste containing bioactive glass particles. Curr Oral Health Rep. 2024;11(2):87–94. https://doi.org/10.1007/s40496-024-00366-3 DOI: https://doi.org/10.1007/s40496-024-00366-3
Jones JR, Brauer DS, Hupa L, Greenspan DC. Bioglass and bioactive glasses and their impact on healthcare. Int J Appl Glass Sci. 2016;7(4):423–34. https://doi.org/10.1111/ijag.12252 DOI: https://doi.org/10.1111/ijag.12252
Jang JH, Lee MG, Ferracane JL, Davis H, Bae HE, Choi D, et al. Effect of bioactive glass-containing resin composite on dentin remineralization. J Dent. 2018;75:58–64. https://doi.org/10.1016/j.jdent.2018.05.017 DOI: https://doi.org/10.1016/j.jdent.2018.05.017
Behzadi S, Mohammadi Y, Rezaei-Soufi L, Farmany A. Occlusion effects of bioactive glass and hydroxyapatite on dentinal tubules: a systematic review. Clin Oral Investig. 2022;26(10):6061–78. https://doi.org/10.1007/s00784-022-04639-y DOI: https://doi.org/10.1007/s00784-022-04639-y
da Cruz LPD, Hill RG, Chen X, Gillam DG. Dentine tubule occlusion by novel bioactive glass-based toothpastes. Int J Dent. 2018;2018(1):5701638. https://doi.org/10.1155/2018/5701638 DOI: https://doi.org/10.1155/2018/5701638
Gesprasert C, Kettratad M, Nimmano N, Wittayanuwat S, Pischom N, Naruphontjirakul P, et al. Effect of a 1.1% NaF toothpaste containing Sr/F-doped bioactive glass on irradiated demineralized dentin: an in vitro study. BMC Oral Health. 2024, 24(1):1399. https://doi.org/10.1186/s12903-024-05186-6 DOI: https://doi.org/10.1186/s12903-024-05186-6
Ciraldo FE, Boccardi E, Melli V, Westhauser F, Boccaccini AR. Tackling bioactive glass excessive in vitro bioreactivity: preconditioning approaches for cell culture tests. Acta Biomater. 2018;75:3–10. https://doi.org/10.1016/j.actbio.2018.05.019 DOI: https://doi.org/10.1016/j.actbio.2018.05.019
Galusková D, Kaňková H, Švančárková A, Buňová L, Galusek D. Direct monitoring of immediate release of Zn from zinc‐doped bioactive glass. Int J Appl Glass Sci. 2022;13(3):476–483. https://doi.org/10.1111/ijag.16568 DOI: https://doi.org/10.1111/ijag.16568
Potiprapanpong W, Naruphontjirakul P, Khamsuk C, Channasanon S, Toneluck A, Tanodekaew S, et al. Assessment of mechanical/chemical properties and cytotoxicity of resin-modified glass ionomer cements containing Sr/F-bioactive glass nanoparticles and methacrylate functionalized polyacids. Int J Mol Sci. 2023;24(12):10231. https://doi.org/10.3390/ijms241210231 DOI: https://doi.org/10.3390/ijms241210231
Chaichana W, Insee K, Chanachai S, Benjakul S, Aupaphong V, Naruphontjirakul P, et al. Physical/mechanical and antibacterial properties of orthodontic adhesives containing Sr-bioactive glass nanoparticles, calcium phosphate, and andrographolide. Sci Rep. 2022;12(1):6635. https://doi.org/10.1038/s41598-022-10654-6 DOI: https://doi.org/10.1038/s41598-022-10654-6
Bruno M, Taddeo F, Medeiros IS, Boaro LC, Moreira MS, Marques MM, et al. Relationship between toothpastes properties and patient-reported discomfort: crossover study. Clin Oral Investig. 2016;20(3):485–94. https://doi.org/10.1007/s00784-015-1539-8 DOI: https://doi.org/10.1007/s00784-015-1539-8
Kim HJ, Bae HE, Lee JE, Park IS, Kim HG, Kwon J, et al. Effects of bioactive glass incorporation into glass ionomer cement on demineralized dentin. Sci Rep. 2021;11(1):7016. https://doi.org/10.1038/s41598-021-86481-y DOI: https://doi.org/10.1038/s41598-021-86481-y
Fu Y, Ekambaram M, Li KC, Zhang Y, Cooper PR, Mei ML. In vitro models used in cariology mineralisation research – a review of the literature. Dent J (Basel). 2024;12(10):323. https://doi.org/10.3390/dj12100323 DOI: https://doi.org/10.3390/dj12100323
British Standard. BS ISO 23317:2014 Implants for surgery. In: In vitro evaluation for apatite-forming ability of implant materials. vol. BS ISO 23317:2014. BSI Standards Limited: London; 2014.
Otsuka Y. Synthesis of hydroxyapatite: crystal growth mechanism and its relevance in drug delivery applications. In: Choi AH, Ben-Nissan B, editors. Innovative bioceramics in translational medicine. 1st ed. Singapore: Springer Singapore; 2022. p. 213–29. DOI: https://doi.org/10.1007/978-981-16-7435-8_7
Vidal Bde C, Mello ML. Collagen type I amide I band infrared spectroscopy. Micron. 2011;42(3):283–9. https://doi.org/10.1016/j.micron.2010.09.010 DOI: https://doi.org/10.1016/j.micron.2010.09.010
Srisomboon S, Kettratad M, Stray A, Pakawanit P, Rojviriya C, Patntirapong S, et al. Effects of silver diamine nitrate and silver diamine fluoride on dentin remineralization and cytotoxicity to dental pulp cells: an in vitro study. J Funct Biomater. 2022;13(1):16. https://doi.org/10.3390/jfb13010016 DOI: https://doi.org/10.3390/jfb13010016
Srisomboon S, Intharah T, Jarujareet U, Toneluck A, Panpisut P. The in vitro assessment of rheological properties and dentin remineralization of saliva substitutes containing propolis and aloe vera extracts. PLoS One. 2024;19(5):e0304156. https://doi.org/10.1371/journal.pone.0304156 DOI: https://doi.org/10.1371/journal.pone.0304156
Seuntjens MT, Thomassen TMJA, Van der Weijden FG., Slot DE. Plaque scores after 1 or 2 minutes of toothbrushing – A systematic review and meta-analysis. Int J Dent Hygiene. 2024; 00: 1-11. https://doi.org/10.1111/idh.12840 DOI: https://doi.org/10.1111/idh.12840
Birant S, Duran Y, Akkoc T, Seymen F. Cytotoxic effects of different detergent containing children’s toothpastes on human gingival epithelial cells. BMC Oral Health. 2022;22(1):66. https://doi.org/10.1186/s12903-022-02089-2 DOI: https://doi.org/10.1186/s12903-022-02089-2
Goenka S, Lee HM. Effect of commercial children’s mouthrinses and toothpastes on the viability of neonatal human melanocytes: an in vitro study. Dent J (Basel). 2023;11(12):287. https://doi.org/10.3390/dj11120287 DOI: https://doi.org/10.3390/dj11120287
Rode SM, Sato TDP, Matos FS, Correia AMO, Camargo SEA. Toxicity and effect of whitening toothpastes on enamel surface. Braz Oral Res. 2021;35:e025. https://doi.org/10.1590/1807-3107bor-2021.vol35.0025 DOI: https://doi.org/10.1590/1807-3107bor-2021.vol35.0025
Katkanchano N, Rirattanapong P, Yimcharoen V. Effect of fluoride-incorporated bioactive glass toothpaste on remineralization of primary enamel lesions: an in-vitro study. J Int Soc Prev Community Dent. 2024;14(6):445–52. https://doi.org/10.4103/jispcd.jispcd_76_24 DOI: https://doi.org/10.4103/jispcd.jispcd_76_24
Poopirom C, Yimcharoen V, Rirattanapong P. Comparative analysis of application of fluoride bioactive glass and sodium fluoride toothpastes for remineralization of primary tooth enamel lesions. J Int Soc Prev Community Dent. 2025;15(1):34–41. https://doi.org/10.4103/jispcd.jispcd_42_24 DOI: https://doi.org/10.4103/jispcd.jispcd_42_24
Wadhawan S, Jain A, Nayyar J, Mehta SK. Role of nanomaterials as adsorbents in heavy metal ion removal from waste water: a review. J Water Process Eng. 2020;33:101038. https://doi.org/10.1016/j.jwpe.2019.101038 DOI: https://doi.org/10.1016/j.jwpe.2019.101038
Guesmi A, Hamadi NB, El-Fattah WA, Subaihi A, Alluhaybi AA, El-Desouky MG, et al. Efficient removal of Pb(II) ions from wastewater via a vanadium metal-organic framework encapsulated with biopolymer carboxymethyl cellulose/polyethylenimine through synthesis, characterization, and Box-Behnken optimization. Int J Biol Macromol. 2025;318:145201. https://doi.org/10.1016/j.ijbiomac.2025.145201 DOI: https://doi.org/10.1016/j.ijbiomac.2025.145201
Tschoppe P, Meyer-Lueckel H, Kielbassa AM. Effect of carboxymethylcellulose-based saliva substitutes on predemineralised dentin evaluated by microradiography. Arch Oral Biol. 2008;;53(3):250–6. https://doi.org/10.1016/j.archoralbio.2007.10.001 DOI: https://doi.org/10.1016/j.archoralbio.2007.10.001
Hai J, Tan X, Yang S, Chen F, Li T, Yang X, et al. Facile preparation of a Ca(ii) carboxymethyl cellulose complex with enhanced calcium bioavailability for treatment of osteoporosis. Dalton Trans. 2019;48(17):5735–40. https://doi.org/10.1039/C9DT00202B DOI: https://doi.org/10.1039/C9DT00202B
Rossler S, Unbehau R, Gemming T, Kruppke B, Wiesmann HP, Hanke T. Calcite incorporated in silica/collagen xerogels mediates calcium release and enhances osteoblast proliferation and differentiation. Sci Rep. 2020;10(1):118. https://doi.org/10.1039/C9DT00202B DOI: https://doi.org/10.1038/s41598-019-56023-8
Wang S, Sheng X, Huang G, Li Q, Dong Y. Dentin remineralization induced by nanobioactive glass in association with RGDS peptide. Mater Today Commun. 2021;28:102515. https://doi.org/10.1016/j.mtcomm.2021.102515 DOI: https://doi.org/10.1016/j.mtcomm.2021.102515
Lee J, Hwang G, Gug H, Lee JH, Park SJ, Park JC. Desensitizing toothpastes for dentin sealing and tertiary dentin formation in vitro and in vivo: a comparative analysis. BMC Oral Health. 2022;22(1):483. https://doi.org/10.1186/s12903-022-02558-8 DOI: https://doi.org/10.1186/s12903-022-02558-8
Park S-R, Kim Y-M, Choi B-B, Kim J-Y. The effect of the cytotoxicity of sodium lauryl sulfate containing toothpaste on HaCaT and NIH-3T3 cells. J Korean Soc Dent Hyg. 2015;15(4):719–25. https://doi.org/10.13065/jksdh.2015.15.04.719 DOI: https://doi.org/10.13065/jksdh.2015.15.04.719
Green A, Crichard S, Ling-Mountford N, Milward M, Hubber N, Platten S, et al. A randomised clinical study comparing the effect of Steareth 30 and SLS containing toothpastes on oral epithelial integrity (desquamation). J Dent. 2019;80(Suppl 1):S33–9. https://doi.org/10.1016/j.jdent.2018.11.005 DOI: https://doi.org/10.1016/j.jdent.2018.11.005
British Standard. Biological evaluation of medical devices Part 5: Tests for in vitro cytotoxicity (ISO 10993–5:2009). London: British Standards; 2009.
Anand A, Sengupta S, Galusek D, Beltran AM, Galuskova D, Boccaccini AR. A new approach to overcome cytotoxic effects of Cu by delivering dual therapeutic ions (Sr, Cu). J Trace Elem Med Biol 2025;87:127565. https://doi.org/10.1016/j.jtemb.2024.127565 DOI: https://doi.org/10.1016/j.jtemb.2024.127565
Sasaki JI, Kiba W, Abe GL, Katata C, Hashimoto M, Kitagawa H, et al. Fabrication of strontium-releasable inorganic cement by incorporation of bioactive glass. Dent Mater. 2019;35(5):780–8. https://doi.org/10.1016/j.dental.2019.02.019 DOI: https://doi.org/10.1016/j.dental.2019.02.019
Sharifianjazi F, Moradi M, Abouchenari A, Pakseresht AH, Esmaeilkhanian A, Shokouhimehr M, et al. Effects of Sr and Mg dopants on biological and mechanical properties of SiO2–CaO–P2O5 bioactive glass. Ceramics Int 2020;46(14):22674–82. https://doi.org/10.1016/j.ceramint.2020.06.030 DOI: https://doi.org/10.1016/j.ceramint.2020.06.030
Silva AV, Gomes DDS, Victor RS, Santana LNL, Neves GA, Menezes RR. Influence of strontium on the biological behavior of bioactive glasses for bone regeneration. Materials (Basel). 2023;16(24):7654. https://doi.org/10.3390/ma16247654 DOI: https://doi.org/10.3390/ma16247654
Lee JH, Mandakhbayar N, El-Fiqi A, Kim HW. Intracellular co-delivery of Sr ion and phenamil drug through mesoporous bioglass nanocarriers synergizes BMP signaling and tissue mineralization. Acta Biomater. 2017;60:93–108. https://doi.org/10.1016/j.actbio.2017.07.021 DOI: https://doi.org/10.1016/j.actbio.2017.07.021
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