Antimicrobial efficacy of light-cured glass ionomer cements incorporating red propolis extract
DOI:
https://doi.org/10.2340/biid.v12.44895Keywords:
Glass ionomer cements, propolis, antimicrobial actionAbstract
Objective: The aim of the present study was to evaluate the antimicrobial activity of resin-modified glass ionomer cements (RMGICs) supplemented with red propolis ethanolic extract (RPEE) against Streptococcus mutans cultures.
Materials and methods: Four RMGICs (Gold Label, Ionolux, Vitro Fill, and Riva) were used with the addition of RPEE at concentrations of 11% and 20%. For the control groups, the cements were manipulated and light-cured according to the manufacturers’ instructions. S. mutans strains from stock cultures were grown in brain heart infusion (BHI) broth. Cement samples were placed in direct contact with the microorganism for 48 h in a bacteriological incubator. After this period, the diameters of the inhibition zones were measured using a digital caliper.
Results: Data analysis showed a significant increase in the size of the inhibition zones for Gold Label and Vitro Fill RMGICs in the groups with 11% RPEE, compared to their respective controls. When comparing different cement brands containing 11% RPEE, Gold Label and Vitro Fill exhibited significantly larger inhibition zones than Ionolux.
Conclusion: The addition of 11% RPEE was shown to enhance the antimicrobial activity of two of the four tested RMGICs, with Gold Label and Vitro Fill demonstrating the greatest antimicrobial potential following supplementation with RPEE.
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Sidhu SK, Nicholson JW. A review of glass-ionomer cements for clinical dentistry. J Funct Biomater. 2016;7(3):16. http://doi.org/10.3390/jfb7030016 DOI: https://doi.org/10.3390/jfb7030016
Moheet IA, Luddin N, Rahman IA, Kannan TP, Nik Abd Ghani NR, Masudi SM. Modifications of glass ionomer cement powder by addition of recently fabricated nano-fillers and their effect on the properties: a review. Eur J Dent. 2019;13(3):470–7. http://doi.org/10.1055/s-0039-1693524 DOI: https://doi.org/10.1055/s-0039-1693524
Meneses IHC, Sampaio GAM, Carvalho FG, Carlo HL, Münchow EA, Pithon MM, et al. In vivo biocompatibility, mechanical, and antibacterial properties of cements modified with propolis in different concentrations. Eur J Dent. 2020;14(1):77–84. http://doi.org/10.1055/s‑0040‑1702255 DOI: https://doi.org/10.1055/s-0040-1702255
Sidhu SK, Watson TF. Resin-modified glass ionomer materials: a status report for the American Journal of Dentistry. Am J Dent. 1995;8(1):59–67. http://doi.org/10.1177/00220345980770091101 DOI: https://doi.org/10.1177/00220345980770091101
Cardoso MV, Delmé KI, Mine A, Neves AA, Coutinho E, De Moor RJ, et al. Towards a better understanding of the adhesion mechanism of resin-modified glass-ionomers by bonding to differently prepared dentin. J Dent. 2010;38:921–9. http://doi.org/10.1016/j.jdent.2010.08.009 DOI: https://doi.org/10.1016/j.jdent.2010.08.009
Almuhaiza M. Glass-ionomer cements in restorative dentistry: a critical appraisal. J Contemp Dent Pract. 2016;17(4):331–6. http://doi.org/10.5005/jp-journals-10024-1850 DOI: https://doi.org/10.5005/jp-journals-10024-1850
Hatunoglu E, Ozturk F, Bilenler T, Aksakalli S, Simsek N. Antibacterial and mechanical properties of propolis added to glass ionomer cement. Angle Orthod. 2014;84:368–73. http://doi.org/10.2319/020413‑101.1 DOI: https://doi.org/10.2319/020413-101.1
Marti LM, Mata M, Ferraz-Santos B, Azevedo ER, Giro EM, Zuanon AC. Addition of chlorhexidine gluconate to a glass ionomer cement: a study on mechanical, physical and antibacterial properties. Braz Dent J. 2014;25:33–7. http://doi.org/10.1590/0103-6440201302328 DOI: https://doi.org/10.1590/0103-6440201302328
De Morais Sampaio GA, Lacerda-Santos R, Cavalcanti YW, Vieira GHA, Nonaka CFW, Alves PM. Antimicrobial properties, mechanics, and fluoride release of ionomeric cements modified by red propolis. Angle Orthod. 2021;91(4):522–7. http://doi.org/10.2319/083120‑759.1 DOI: https://doi.org/10.2319/083120-759.1
Akao Y, Maruyama H, Matsumoto K, et al. Cell growth inhibitory effect of cinnamic acid derivatives from propolis on human tumor cell lines. Biol Pharm Bull. 2003;26(7):1057–9. http://doi.org/ 10.1248/bpb.26.1057 DOI: https://doi.org/10.1248/bpb.26.1057
Ferreira FB, Torres SA, Rosa OP, et al. Antimicrobial effect of propolis and other substances against selected endodontic pathogens. Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2007;104(5):709–16. http://doi.org/10.1016/j.tripleo.2007.05.019 DOI: https://doi.org/10.1016/j.tripleo.2007.05.019
Jafarzadeh Kashi TS, Kasra Kermanshahi R, Erfan M, Vahid Dastjerdi E, Rezaei Y, Tabatabaei FS. Evaluating the in vitro antibacterial effect of Iranian propolis on oral microorganisms. Iran J Pharm Res. 2011;10(2):363–8. http://doi.org/10.22037/ijpr.2011.967
Esmeraldo MR, Carvalho MG, Carvalho RA, Lima RF, Costa EM. Inflammatory effect of green propolis on dental pulp in rats. Braz Oral Res. 2013;27(5):417–22. http://doi.org/10.1590/S1806-83242013005000022 DOI: https://doi.org/10.1590/S1806-83242013005000022
Grenho L, Barros J, Ferreira C, Santos VR, Monteiro FJ, Ferraz MP, et al. In vitro antimicrobial activity and biocompatibility of propolis containing nanohydroxyapatite. Biomed Mater. 2015;10:025001. http://doi.org/10.1088/1748-6041/10/2/025004 DOI: https://doi.org/10.1088/1748-6041/10/2/025004
Andrade JKS, Denadai M, de Oliveira CS, Nunes ML, Narain N. Evaluation of bioactive compounds potential and antioxidant activity of brown, green and red propolis from Brazilian northeast region. Food Res Int. 2017;101:129–38. http://doi.org/10.1016/j.foodres.2017.08.066 DOI: https://doi.org/10.1016/j.foodres.2017.08.066
Sampaio GAM, Lacerda-Santos R, Cavalcanti YW, Vieira GHA, Nonaka CFW, Alves PM. Biocompatibility of ionomeric cements modified by red propolis: a morphological and immunohistochemical analysis. J Adhes Dent. 2020;22(5):515–22. http://doi.org/10.3290/j.jad.a36512
Elghazouly YM, Amer AN. Studying the effect of egyptian propolis on antimicrobial properties of glass ionomer cement. J Adv Pharm Res. 2021;5(3):336–40. http://doi.org/10.21608/aprh.2021.74157.112
Saputra SK, Sutantyo D, Farmasyanti CA, Alhasyimi AA. The effect of the addition of propolis to resin modified glass ionomer cement bracket adhesive materials on the growth inhibition zone of Streptococcus mutans. F1000Res. 2019;8:2105. http://doi.org/10.12688/f1000research.20717.2 DOI: https://doi.org/10.12688/f1000research.20717.1
Panahandeh N, Adinehlou F, Sheikh-Al-Eslamian SM, Torabzadeh H. Extract of propolis on resin-modified glass ionomer cement: effect on mechanical and antimicrobial properties and dentin bonding strength. Int J Biomater. 2021;2021:1–8. http://doi.org/10.1155/2021/5597837 DOI: https://doi.org/10.1155/2021/5597837
Udoye CI, Jafarzadeh H, Abbott PV. Transport media for avulsed teeth: a review. Aust Endod J. 2012;38(3):129–36. http://doi.org/10.1111/j.1747-4477.2012.00356.x DOI: https://doi.org/10.1111/j.1747-4477.2012.00356.x
Fúcio SB, Paula AB, Sardi JC, Duque C, Correr-Sobrinho L, Puppin-Rontani RM. Streptococcus mutans biofilm influences on the antimicrobial properties of glass ionomer cements. Braz Dent J. 2016;27(6):681–7. http://doi.org/10.1590/0103‑6440201600431 DOI: https://doi.org/10.1590/0103-6440201600655
Pingueiro JMS, Feres MFN, Vaz-Monteiro CCM, Vieira-Silva HK, Vargas GQ, Malavazi LM, et al. Brazilian red propolis as a potential antimicrobial additive in orthodontic primers. Braz Dent J. 2025;36:e246036 http://doi.org/10.1590/0103-644020256036 DOI: https://doi.org/10.1590/0103-644020256036
Neto NFO, Bonvicini JFS, de Souza GL, Santiago MB, Veneziani RCS, Ambrósio SR, et al. Antibacterial activity of Brazilian red propolis and in vitro evaluation of free radical production. Arch Oral Biol. 2022;143:105520. http://doi.org/10.1016/j.archoralbio.2022.105520 DOI: https://doi.org/10.1016/j.archoralbio.2022.105520
Sagmak S, Bahsi E, Ozcan N, Satici O. Comparative evaluation of antimicrobial efficacy and fluoride release of seven different glass-ionomer-based restorative materials. Oral Health Prev Dent. 2020;18(3):521–8. http://doi.org/10.3290/j.ohpd.a44140
Shashibhushan KK, Basappa N, Subba Reddy VV. Comparison of antibacterial activity of three fluorides- and zinc-releasing commercial glass ionomer cements on strains of mu-tans streptococci: an in vitro study. J Indian Soc Pedod Prev Dent. 2008;26 Suppl 2:S56–61. http://doi.org/10.4103/0970‑4388.95580 DOI: https://doi.org/10.4103/0970-4388.43533
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