Sealing ability of Biodentine, zirconia reinforced glass ionomer cement and Mineral Trioxide Aggregate as furcation perforation repair materials: an in vitro analysis
DOI:
https://doi.org/10.2340/biid.v13.45038Keywords:
Biodentine, Mineral Trioxide Aggregate angelus, Zirconia Reinforced Glass Ionomer cement, furcation perforation repairAbstract
Aim: The present study aimed to evaluate and compare the sealing ability of Biodentine, zirconia reinforced glass ionomer cement (GIC), and Mineral Trioxide Aggregate (MTA) as furcation repair materials.
Materials and methods: A total of 50 extracted permanent maxillary molars were collected and divided into three experimental groups and one control group. Group I – Biodentine (n = 15), Group II – zirconia reinforced GIC (n = 15), Group III – MTA Angelus (n = 15), and unrepaired, control group (n = 5). Crowns of teeth in experimental groups were sectioned 3 mm above the cementoenamel junction and roots 3 mm below the furcation. Standardised endodontic access openings were prepared, canal orifices and root ends were sealed with sticky wax. After coating with nail varnish, a 1 mm furcation perforation was created at a standardised location using a round carbide bur. Samples were flushed, dried, and incubated at 37°C for 24 h to simulate clinical conditions. All samples were subjected to orthograde and retrograde methylene blue dye challenge followed by dye extraction with a concentration of 65% nitric acid. Samples were then analysed using 550 ultraviolet-visible spectrophotometers.
Statistical analysis: The results were analysed statistically by one-way analysis of variance (ANOVA) and Tukey’s multiple comparison tests.
Result: No statistically significant difference in sealing ability was observed between Biodentine, zirconia reinforced GIC, and MTA when used as a furcation perforation repair material.
Conclusion: Within the limitations of this study, it can be concluded that Biodentine, zirconia reinforced GIC, and MTA showed sealing ability comparable to each other.
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Balachandran J, Gurucharan. Comparison of sealing ability of bioactive bone cement, Mineral Trioxide Aggregate and Super EBA as furcation repair materials: a dye extraction study. J Conserv Dent. 2013;16:247–51. https://doi.org/10.4103/0972-0707.111326 DOI: https://doi.org/10.4103/0972-0707.111326
Ingle JL. A standardized endodontic technique utilizing newly designed instruments and filling materials. Oral Surg Oral Med Oral Pathol. 1961;14: 83–91. https://doi.org/10.1016/0030-4220(61)90477-7 DOI: https://doi.org/10.1016/0030-4220(61)90477-7
Garg AK, Aggarwal P, Singh BP, Kumari M. Accidental root perforations: prevalence and implications during endodontic treatment. Int Endod J. 2022;55(4):320–8.
Eleftheriadis GI, Lambrianidis TP. Technical quality of root canal treatment and detection of iatrogenic errors in an undergraduate dental clinic. Int Endod J. 2005;38:725–34. https://doi.org/10.1111/j.1365-2591.2005.01008.x DOI: https://doi.org/10.1111/j.1365-2591.2005.01008.x
American Association of Endodontists. Glossary of Endodontic terms. 7th ed. Chicago, IL: American Association of Endodontics; 2003.
Gagliani MM. The outcome of endodontic retreatment: a 2-yr follow-up. J Endod. 2004;30:1–4. https://doi.org/10.1097/00004770-200401000-00001 DOI: https://doi.org/10.1097/00004770-200401000-00001
Benenati FW, Roane JB, Biggs JT, Simon JH. Recall evaluation of iatrogenic root perforations repaired with amalgam and gutta-percha. J Endod. 1986;12:161–6. https://doi.org/10.1016/S0099-2399(86)80055-3 DOI: https://doi.org/10.1016/S0099-2399(86)80055-3
Yaltırık M, Ozbas H, Bilgic B, Issever H, Reznikov N. Evaluation of microleakage and cytotoxicity of a new root-end filling material in different dilutions. J Endod. 2006;32:833–6.
Wu MK, Wesselink PR. Endodontic leakage studies reconsidered Part I Methodology, application and relevance. Int Endod J. 1993;26:37–43. https://doi.org/10.1111/j.1365-2591.1993.tb00540.x DOI: https://doi.org/10.1111/j.1365-2591.1993.tb00540.x
Priyalakshmi S, Ranjan M. Review of Biodentine – a bioactive dentin substitute. IOSR J Dent Med Sci. 2014;13:13–7. https://doi.org/10.9790/0853-13131317 DOI: https://doi.org/10.9790/0853-13131317
Han L, Okiji T. Uptake of calcium and silicon released from calcium silicate-based endodontic materials into root canal dentine. Int Endod J. 2011;44:1081–7. https://doi.org/10.1111/j.1365-2591.2011.01924.x
Torabeinejad M, Pitt Ford TR. Antibacterial effects of some root end filling material. Am Assoc Endodon. 1995;21:403–6. https://doi.org/10.1016/S0099-2399(06)80824-1 DOI: https://doi.org/10.1016/S0099-2399(06)80824-1
Tawil PZ, Duggan DJ, Galicia JC. Mineral Trioxide Aggregate (MTA): its history, composition, and clinical applications. Compend Contin Educ Dent. 2015;36:247–52.
Walia R, Jasuja P, Verma KG, Juneja S, Mathur A, Ahuja L, et al. A comparative evaluation of microleakage and compressive strength of Ketac Molar, Giomer, Zirconomer, and Ceram-x: an in vitro study. J Indian Soc Pedod Prev Dent. 2016;34:280–4. https://doi.org/10.4103/0970-4388.186746 DOI: https://doi.org/10.4103/0970-4388.186746
Patel DM, Patel JJ. Comparison of apical microleakage of bioceramic sealers & epoxy resin-based sealer using dye extraction leakage method. Int J Sci Res. 2023;12(7):1–3.
Alshehri MM, Alhawsawi BF, Alghamdi A, Aldobaikhi SO, Alanazi MH, Alahmad FA. The management of root perforation: a review of the literature. Cureus.2024;24:16–24. https://doi.org/10.7759/cureus.72296 DOI: https://doi.org/10.7759/cureus.72296
Muliyar S, Shameem KA, Thankachan RP, Francis PG, Jayapalan CS, Hafiz KA. Microleakage in endodontics. J Int Oral Health. 2014;6:99–104.
Gogna R, Jagadis S, Shashikal K. A comparative in vitro study of microleakage by a radioactive isotope and compressive strength of three nanofilled composite resin restorations. J Conserv Dent. 2011;14:128–31. https://doi.org/10.4103/0972-0707.82609 DOI: https://doi.org/10.4103/0972-0707.82609
Camps J, Pashley D. Reliability of the dye penetration studies. J Endod. 2003;29:592–4. https://doi.org/10.1097/00004770-200309000-00012 DOI: https://doi.org/10.1097/00004770-200309000-00012
Torabinejad M, Watson TF, Pitt Ford TR. Sealing ability of a Mineral Trioxide Aggregate when used as a root end filling material. J Endod. 1993;19:591–5. DOI: https://doi.org/10.1016/S0099-2399(06)80271-2
Latheef MA, Al‐Madi EM, Duggal MS, Al‐Wamiddle H. Apical microleakage assessment of root‑end filling materials using dye extraction and spectrophotometric analysis. J Conserv Dent. 2019;22:603–7.
Fayed MB, Ibrahim MM, Hassanein KM. Comparative in vitro evaluation of microleakage of bioceramic, resin-based, and calcium hydroxide root canal sealers using spectrophotometric dye penetration technique. F1000Research. 2022;11:1396. https://doi.org/10.12688/f1000research.127911.1 DOI: https://doi.org/10.12688/f1000research.127911.1
Oppenheimer S, Rosenberg PA. Effect of temperature change on the sealing properties of Cavit and Cavit G. Oral Surg Oral Med oral Pathol. 1979;48:250–3. https://doi.org/10.1016/0030-4220(79)90012-4 DOI: https://doi.org/10.1016/0030-4220(79)90012-4
Grech L, Mallia B, Camilleri J. Investigation of the physical properties of tricalcium silicate cement-based root-end filling materials. Dent Mater J. 2015;34(6):654–9.
Gudapati S, Satish RK, Sajjan GS, Varma KM, Kumar VS. Comparative evaluation of blood contamination on the push-out bond strength of Mineral Trioxide Aggregate, Biodentine, and bone cement in furcation perforation repair: an in vitro study. Endodontology. 2024;36(4):346–52. https://doi.org/10.4103/endo.endo_52_24 DOI: https://doi.org/10.4103/endo.endo_52_24
Usta SN, Keskin C. Color stability and solubility of Biodentine and NeoPutty in contact with different irrigation solutions. Restor Dent Endod. 2024;49(3):e25. https://doi.org/10.5395/rde.2024.49.e25 DOI: https://doi.org/10.5395/rde.2024.49.e25
Han L, Okiji T. Uptake of calcium and silicon released from calcium silicate–based endodontic materials into root canal dentine. Int Endodon J. 2011;44:1081–7. https://doi.org/10.1111/j.1365-2591.2011.01924.x DOI: https://doi.org/10.1111/j.1365-2591.2011.01924.x
Singla M, Verma KG, Goyal V, Jusuja P, Kakkar A, Ahuja L. Comparison of push-out bond strength of furcation perforation repair materials – glass ionomer cement type II, hydroxyapatite, Mineral Trioxide Aggregate, and Biodentine: an in vitro Study. Contemp ClinDent. 2018;9:410–4. https://doi.org/10.4103/ccd.ccd_162_18 DOI: https://doi.org/10.4103/ccd.ccd_162_18
Torabinejad M. Mineral Trioxide Aggregate: a comprehensive
literature review Part II: leakage and biocompatibility. J Endod. 2010;36:190–202. https://doi.org/10.1016/j.joen.2009.09.010 DOI: https://doi.org/10.1016/j.joen.2009.09.010
Chaudhari PS, Chandak MG, Jaiswal AA, Ikhar A. Comparative evaluation of push-out bond strength of three retrograde filling materials in teeth with root apices resected at 90°: Mineral Trioxide Aggregate Angelus, Zirconomer, and Bioactive bone cement. J Conserv Dent. 2022;25:605–9. DOI: https://doi.org/10.4103/jcd.jcd_268_22
Camilleri J, Sorrentino F, Damidot D. Investigation of the hydration and bioactivity of radiopacified tricalcium silicate cement, Biodentine and MTA Angelus. Dent Mater. 2013;29:580–593. https://doi.org/10.1016/j.dental.2013.03.007 DOI: https://doi.org/10.1016/j.dental.2013.03.007
Seltzer S, Sinai I, August D. Periodontal effects of root perforations before and during endodontic procedures. J Dent Res. 1970;49:332–9. https://doi.org/10.1177/00220345700490022301 DOI: https://doi.org/10.1177/00220345700490022301
Kaur M, Singh H, Dhillon JS, Batra M, Saini M. MTA versus Biodentine: review of literature with a comparative analysis. J Clin Diagn Res. 2017;11:1–5. https://doi.org/10.7860/JCDR/2017/25840.10374 DOI: https://doi.org/10.7860/JCDR/2017/25840.10374
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