Food dye staining of dental composites: material variability and mouthwash-induced staining reversibility

Burçin Deniz; Osman Tolga Harorli

doi:10.2340/biid.v13.45823

Authors

Burçin Deniz Department of Restorative Dentistry, Faculty of Dentistry, Akdeniz University, Antalya, Turkey https://orcid.org/0000-0003-0386-4261
Osman Tolga Harorli Department of Restorative Dentistry, Faculty of Dentistry, Akdeniz University, Antalya, Turkey https://orcid.org/0000-0001-6828-5063

DOI:

https://doi.org/10.2340/biid.v13.45823

Keywords:

resin based composites, food colouring agents, colour stability, mouthwashes, spectrophotometry

Abstract

Objective: Food dyes, widely used in consumables, may interact with polymeric materials, yet their staining effects on resin based dental composites remain underexplored. This in vitro study investigates the staining potential of five common water-soluble food dyes on contemporary dental composites and evaluates the efficacy of Listerine Advanced White mouthwash in reversing discolouration, using both colour difference and whiteness-based analyses.

Methods: Five synthetic dyes – Tartrazine, Sunset Yellow, Ponceau 4R, Carmoisine, and Brilliant Blue – were tested on six resin composites: Charisma Smart, Estelite ∑ Quick, Filtek One Bulkfill, RubyFlow, Spectra SphereTEC High Viscosity (STHV), and Filtek Ultimate. Sixty disk-shaped samples (7 mm × 2 mm) per composite (N = 360) were immersed in dye solutions (50 mg/L) or distilled water (control) for 7 days. Colour changes were evaluated using the CIEDE2000 colour difference formula (ΔE₀₀) and the dental whiteness index (WID), calculated from Commission Internationale de l’Eclairage L*a*b* color space (CIELAB) coordinates obtained with a spectrophotometer.

Results: Brilliant Blue exhibited the highest staining potential, producing ΔE₀₀ values exceeding the clinically unacceptable threshold (1.8) in all composites except Spectra STHV, and was also associated with the greatest changes in WID values. Other dyes caused variable and generally less pronounced changes in both ΔE₀₀ and WID. The whitening mouthwash partially reversed discolouration, reflected by significant increases in WID and reductions in ΔE₀₀, with significant recovery observed in Estelite, RubyFlow, and Filtek One (p < 0.05). Analysis of variance revealed significant effects of dye type, composite material, and treatment on both ΔE₀₀ and WID outcomes (p < 0.001).
Conclusion: The colour stability and whiteness of dental resin composites are affected by synthetic food dyes in a material- and dye-dependent manner. Whitening mouthwash can partially restore both colour difference and whiteness; however, complete recovery to baseline values is generally not achieved.

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References

Saini RS, Bindu Hayyim RIH, Gurumurthy V, Alshadidi AAF, Aldosari LIN, Okshah A, et al. Dental biomaterials redefined: molecular docking and dynamics-driven dental resin composite optimization. BMC Oral Health. 2024;24:557.

https://doi.org/10.1186/s12903-024-04343-1 DOI: https://doi.org/10.1186/s12903-024-04343-1

Cinelli F, Russo DS, Nieri M, Giachetti L. Stain susceptibility of composite resins: pigment penetration analysis. Materials. 2022;15:4874.

https://doi.org/10.3390/ma15144874 DOI: https://doi.org/10.3390/ma15144874

Islam MS, Aal-Fatlah AA, Alkhan NS, Aryal Ac S, Sadr A, Rehman MM. The effect of different finishing polishing protocols on stain absorption and color stability of resin composite restorations. Am J Dent. 2022;35:141–5.

Zhao X, Zanetti F, Wang L, Pan J, Majeed S, Malmstrom H, et al. Effects of different discoloration challenges and whitening treatments on dental hard tissues and composite resin restorations. J Dent. 2019;89:103182.

https://doi.org/10.1016/j.jdent.2019.103182 DOI: https://doi.org/10.1016/j.jdent.2019.103182

Lis K, Bartuzi Z. Plant food dyes with antioxidant properties and allergies-friend or enemy? Antioxidants. 2023;12:1357.

https://doi.org/10.3390/antiox12071357 DOI: https://doi.org/10.3390/antiox12071357

Alegbe EO, Uthman TO. A review of history, properties, classification, applications and challenges of natural and synthetic dyes. Heliyon. 2024;10:e33646.

https://doi.org/10.1016/j.heliyon.2024.e33646 DOI: https://doi.org/10.1016/j.heliyon.2024.e33646

Hamdy TMM, Abdelnabi A, Othman MSS, Bayoumi REE, Abdelraouf RMM. Effect of different mouthwashes on the surface microhardness and color stability of dental nanohybrid resin composite. Polymers. 2023;15:815.

https://doi.org/10.3390/polym15040815 DOI: https://doi.org/10.3390/polym15040815

Sultan MS. Effect of hydrogen peroxide versus charcoal-based whitening mouthwashes on color, surface roughness, and color stability of enamel. BMC Oral Health. 2024;24:897.

https://doi.org/10.1186/s12903-024-04631-w DOI: https://doi.org/10.1186/s12903-024-04631-w

Naidu AS, Bennani V, Aarts JM, Brunton P. Over-the-counter tooth whitening agents: a review of literature. Braz Dent J. 2020;31:221–35.

https://doi.org/10.1590/0103-6440202003227 DOI: https://doi.org/10.1590/0103-6440202003227

Walters PA, Biesbrock AR, Bartizek RD. Benefits of sodium hexametaphosphate-containing chewing gum for extrinsic stain inhibition. J Dent Hyg. 2004;78:8.

García-Falcón MS, Simal-Gándara J. Determination of food dyes in soft drinks containing natural pigments by liquid chromatography with minimal clean-up. Food Control. 2005;16(3):293–7.

https://doi.org/10.1016/j.foodcont.2004.03.009 DOI: https://doi.org/10.1016/j.foodcont.2004.03.009

Paravina RD, Perez MM, Ghinea R. Acceptability and perceptibility thresholds in dentistry: a comprehensive review of clinical and research applications. J Esthet Restor Dent. 2019;31:103–12.

https://doi.org/10.1111/jerd.12465 DOI: https://doi.org/10.1111/jerd.12465

del Mar Pérez M, Ghinea R, Rivas MJ, Yebra A, Ionescu AM, Paravina RD, et al. Development of a customized whiteness index for dentistry based on CIELAB color space. Dent Mater. 2016;32(30):461–7.

https://doi.org/10.1016/j.dental.2015.12.008 DOI: https://doi.org/10.1016/j.dental.2015.12.008

Topolska J, Motyl S, Orłowska A, Borkowski A, Działak P, Gronkiewicz K. The effect of brilliant blue-based plaque-staining agents on aesthetic orthodontic appliances. Materials. 2021;14:7050.

https://doi.org/10.3390/ma14227050 DOI: https://doi.org/10.3390/ma14227050

Joiner A. Tooth colour: a review of the literature. J Dent. 2004;32:3–12.

https://doi.org/10.1016/j.jdent.2003.10.013 DOI: https://doi.org/10.1016/j.jdent.2003.10.013

Ntovas P, Masouras K, Lagouvardos P. Efficacy of non-hydrogen peroxide mouthrinses on tooth whitening: an in vitro study. J Esthet Restor Dent. 2021;33:1059–65.

https://doi.org/10.1111/jerd.12800 DOI: https://doi.org/10.1111/jerd.12800

Harorli OT, Barutcigil C. Color recovery effect of commercial mouth rinses on a discolored composite. J Esthet Restor Dent. 2014;26:256–63.

https://doi.org/10.1111/jerd.12107 DOI: https://doi.org/10.1111/jerd.12107

He T, Baker R, Bartizek RD, Biesbrock AR, Chaves E, Terézhalmy G. Extrinsic stain removal efficacy of a stannous fluoride dentifrice with sodium hexametaphosphate. J Clin Dent. 2007;18:7–11.

Oliveira JBS, Sarlo RS, Bresciani E, Caneppele TMF. Whitening efficacy of whitening mouth rinses used alone or in conjunction with carbamide peroxide home whitening. Oper Dent. 2017;42:319–26.

https://doi.org/10.2341/15-361-L DOI: https://doi.org/10.2341/15-361-L

Pedersen A, Sørensen C, Proctor G, Carpenter G. Salivary functions in mastication, taste and textural perception, swallowing and initial digestion. Oral Dis. 2018;24:1399–416.

https://doi.org/10.1111/odi.12867 DOI: https://doi.org/10.1111/odi.12867

El-Rashidy AA, Abdelraouf RM, Habib NA. Effect of two artificial aging protocols on color and gloss of single-shade versus multi-shade resin composites. BMC Oral Health. 2022;22:321.

https://doi.org/10.1186/s12903-022-02351-7 DOI: https://doi.org/10.1186/s12903-022-02351-7

Hayashi K, Kurokawa H, Saegusa M, Aoki R, Takamizawa T, Kamimoto A, et al. Influence of surface roughness of universal shade resin composites on color adjustment potential. Dent Mater J. 2023;42:676–82.

https://doi.org/10.4012/dmj.2023-007 DOI: https://doi.org/10.4012/dmj.2023-007

Fatima T, Abubakar P, Deshpande S, Afreen N, Sheshnag G, Safoora S. Evaluation of the effect of various beverages and food materials on the color stability of provisional materials: an in vitro study. Cureus. 2024;16:e63941.

https://doi.org/10.7759/cureus.63941 DOI: https://doi.org/10.7759/cureus.63941

Irawan BA, Irawan SN, Masudi SM, Sukminingrum N, Alam MK. 3D surface profile and color stability of tooth colored filling materials after bleaching. Biomed Res Int. 2015;2015:327289.

https://doi.org/10.1155/2015/327289 DOI: https://doi.org/10.1155/2015/327289