Effect of dentine moisture conditions on interfacial adaptation of a calcium silicate-based sealer compared with an epoxy resin-based sealer: an SEM/EDX analysis

Authors

  • Maha M. Yahya Department of Conservative Dentistry, College of Dentistry, University of Mustansiriyah, Baghdad, Iraq
  • Baidaa Mohammed Zeidan Department of Conservative Dentistry, College of Dentistry, University of Mustansiriyah, Baghdad, Iraq
  • Njwan Fadhel Shehab Department of Conservative Dentistry, College of Dentistry, University of Mosul, Mosul, Iraq

DOI:

https://doi.org/10.2340/biid.v13.46517

Keywords:

Adhesive interface, dentine moisture, EDX analysis, interfacial adaptation, sealer

Abstract

Introduction: To evaluate the effect of dentine moisture conditions on interfacial adaptation of a calcium silicate-based sealer (Bio-C Sealer) compared with that of an epoxy resin–based sealer (AH Plus Jet) at different root levels.

Methods: Sixty extracted single-rooted teeth were prepared using ProTaper Universal system up to size F3 and randomly allocated to two groups (n = 30) according to the type of sealer. Each group was then further subdivided into three subgroups (n = 10 each) according to dentine moisture condition: dry (ethanol as final irrigant), normal moist (blotted dry), and wet (canals left flooded). Root sections (2 mm in thickness) were obtained at 3 mm (apical) and 8 mm (coronal). Interfacial adaptation was assessed using scanning electron microscopy (SEM) analysis, while elemental composition was analyzed utilizing energy-dispersive X-ray spectroscopy analysis (EDX). Data were analyzed using two-way analysis of variance and Tukey’s post hoc test (p ≤ 0.05).

Results: Dentine moisture significantly affected interfacial adaptation. AH Plus Jet demonstrated superior adaptation under dry conditions, while Bio-C Sealer demonstrated optimal adaptation under moist conditions. EDX analysis revealed significantly higher levels of calcium and phosphorus in the Bio-C Sealer compared with the AH Plus group (p ≤ 0.019; p ≤ 0.036). Within the Bio-C Sealer group, calcium levels were significantly lower coronally (p = 0.016) than apically. Silicon and carbon were higher in AH Plus (p < 0.01), while oxygen was higher in Bio-C (p < 0.001).

Conclusion: Dentine moisture conditions significantly influence the performance of sealer. AH Plus performs optimally under dry conditions, while Bio-C Sealer demonstrates superior performance in moist environments.

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Published

2026-07-16

How to Cite

Yahya, M. M., Zeidan, B. M., & Shehab, N. F. (2026). Effect of dentine moisture conditions on interfacial adaptation of a calcium silicate-based sealer compared with an epoxy resin-based sealer: an SEM/EDX analysis. Biomaterial Investigations in Dentistry, 13(1), 583–592. https://doi.org/10.2340/biid.v13.46517