Protein and mRNA Expression Levels of Interleukin-17A, -17F and -22 in Blood and Skin Samples of Patients with Mycosis Fungoides

Authors

  • Despoina Papathemeli
  • Aikaterini Patsatsi
  • Dimitrios Papanastassiou
  • Triantafyllia Koletsa
  • Thomas Papathemelis
  • Chrysostomos Avgeros
  • Olga Pikou
  • Elizabeth Lazaridou
  • Elisavet Georgiou

DOI:

https://doi.org/10.2340/00015555-3688

Keywords:

mycosis fungoides, primary cutaneous T-cell lymphoma, CTCL, IL-17A, IL-17F, IL-22

Abstract

This study investigated the expression of interleukin (IL)-17A, -17F and -22 in mycosis fungoides. Blood samples were collected from 50 patients with mycosis fungoides and 50 healthy controls. Skin samples were obtained from 26 patients with mycosis fungoides and 5 healthy controls. Protein levels of IL-17A, -17F and -22 were measured in serum by multiplex enzyme-linked immunosorbent assay, and mRNA expression levels were measured in blood and skin samples by real-time quantitative reverse transcription PCR. Both IL-17A and IL-17F mRNA expression levels were significantly lower in blood of patients with mycosis fungoides in comparison with healthy controls. IL-22 serum levels and expression levels of IL-22 mRNA in skin tissue, were significantly increased in patients with mycosis fungoides in comparison with healthy controls. These results suggest that low levels of IL-17A and IL-17F in mycosis fungoides may be connected to impaired immune surveillance contributing to tumourigenesis. Upregulation of IL-22 may play a role in the establishment of the tumour microenvironment in mycosis fungoides.

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Published

2020-11-18

How to Cite

Papathemeli, D., Patsatsi, A., Papanastassiou, D., Koletsa, T., Papathemelis, T., Avgeros, C., Pikou, O., Lazaridou, E., & Georgiou, E. (2020). Protein and mRNA Expression Levels of Interleukin-17A, -17F and -22 in Blood and Skin Samples of Patients with Mycosis Fungoides. Acta Dermato-Venereologica, 100(18), 1–6. https://doi.org/10.2340/00015555-3688

Issue

Vol. 100 No. 18 (2020): November

Section

Articles

License

Copyright (c) 2020 Despoina Papathemeli, Aikaterini Patsatsi, Dimitrios Papanastassiou, Triantafyllia Koletsa, Thomas Papathemelis, Chrysostomos Avgeros, Olga Pikou, Elizabeth Lazaridou, Elisavet Georgiou

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This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

All digitalized ActaDV contents is available freely online. The Society for Publication of Acta Dermato-Venereologica owns the copyright for all material published until volume 88 (2008) and as from volume 89 (2009) the journal has been published fully Open Access, meaning the authors retain copyright to their work.

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