Antigen specific human T cell lines specific for cobalt chloride
DOI:
https://doi.org/10.2340/0001555566200206Abstract
T lymphocyte lines specific for cobalt (CoCl2) were established from one cobalt and nickel sensitive donor. The lines were generated by stimulating peripheral blood mononuclear cells (PBL) with CoCl2 for 7 days. The activated blasts were maintained in interleukin 2 containing media and every 7-10 day restimulated with antigen (CoCl2) and fresh irradiated PBL. The antigen specificity of the lines was attested by their capacity to proliferate and release of IL 2 under restimulation by CoCl2 and not by an antigen like NiSO4, towards which the donor was also sensitized. The cell lines were of the helper phenotype, T3/T4 positive subset, as determined by monoclonal antibodies. Analysis of the HLA class II restriction by using allogeneic PBL as antigen presenting cells and the capacity of anti HLA-D antibodies to effectively inhibit the specific response indicated that the antigen specific T cell response to contact allergens like cobalt chloride is restricted to HLA class II antigens.Downloads
Downloads
Published
How to Cite
Issue
Section
License
Copyright (c) 1986 Acta Dermato-VenereologicaAll digitalized ActaDV contents is available freely online. The Society for Publication of Acta Dermato-Venereologica owns the copyright for all material published until volume 88 (2008) and as from volume 89 (2009) the journal has been published fully Open Access, meaning the authors retain copyright to their work.
Unless otherwise specified, all Open Access articles are published under CC-BY-NC licences, allowing third parties to copy and redistribute the material in any medium or format and to remix, transform, and build upon the material for non-commercial purposes, provided proper attribution to the original work.