Phenotype of cells involved in mycosis fungoides and Sézary syndrome (blood and skin lesions): immunomorphological study with monoclonal antibodies

Abstract

The phenotypic expression of immunocompetent cells involved in lesions of cutaneous T-cell lymphoma (mycosis fungoides and Sézary syndrome) was studied by using monoclonal antibodies specific for T-cell subsets and HLA-DR antigens (OKT3, T4, T6, T8) (BL 6, BL 5, BL 2). In Sézary syndrome, the phenotype of Sézary cell is homogeneous (T3+, T4+, T8-, T6-, BL2-) and corresponds to the phenotype expressed by the normal helper T-cell population. At the ultrastructural level, the discontinuous distribution of OKT4 and OKT3 antigenic sites on the cell surface is similar to the HTLA antigens. In skin lesions, the Langerhans cells expressed two specificities shared by normal thymocytes (T6 and BL6). We suggest that the expression of these antigens could be related to the epithelial microenvironment (epithelium-dependent differentiation antigens). The tumoral lymphoid cells infiltrating the skin showed the same phenotype (helper type) as the circulating Sézary cells (T3+, T4+, T8-, T6-). Moreover, the T3+ cells also expressed HLA-DR antigens and corresponded to activated T lymphocytes. The various subpopulations of immunocompetent cells seemed to be related to the MHC-derived antigens.